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Purified anti-MAP6 (STOP) Antibody (Previously Covance catalog# MMS-5104)

Pricing & Availability
Clone
STOP (See other available formats)
Regulatory Status
RUO
Other Names
Microtubule-associated protein 6, MAP-6, stable tubule-only polypeptide
Previously
Covance Catalog# MMS-5104
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
1_STOP_Purified_MAP6_Anitbody_3_123118.png
Western blot of purified anti-MAP6 (STOP) antibody (clone STOP). Lane 1: Molecular weight marker; Lane 2: 20 µg of rat brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 1_STOP_Purified_MAP6_Anitbody_3_123118.png
    Western blot of purified anti-MAP6 (STOP) antibody (clone STOP). Lane 1: Molecular weight marker; Lane 2: 20 µg of rat brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 2_STOP_Purified_MAP6_Anitbody_1_123118.png
    IHC staining of purified anti-MAP6 (STOP) antibody (clone STOP) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 3_STOP_Purified_MAP6_Anitbody_2_123118.png
    IHC staining of purified anti-MAP6 (STOP) antibody (clone STOP) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
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824701 100 µL £189
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Description

Microtubule-associated protein 6 (MAP6), also known as stable tubule-only polypeptide or STOP, is a calmodulin-binding protein that is involved in microtubule stabilization and is also regulated by calmodulin. MAP6 regulates lysosomal trafficking and is involved in dendrite maintenance and morphogenesis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against the STOP protein derived from the brains of rats.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1 - 5 µg per ml. For immunohistochemistry, a concentration range of 5 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB) and immunohistochemistry (IHC-P).

RRID
AB_2564883 (BioLegend Cat. No. 824701)

Antigen Details

Structure
Expected MW: 145 kD
Biology Area
Cell Biology, Neuroscience, Synaptic Biology
Molecular Family
Postsynaptic proteins
Gene ID
4135 View all products for this Gene ID
UniProt
View information about MAP6 on UniProt.org
Go To Top Version: 4    Revision Date: 07/09/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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