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Purified anti-MAVS Antibody

Pricing & Availability
Clone
W21144C (See other available formats)
Regulatory Status
RUO
Other Names
Interferon beta promoter stimulator protein 1, IPS-1, Virus-induced-signaling adapter, VISA, Mitochondrial Antiviral-Signaling Protein
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W21144C_PURE_MAVS_Antibody_1_010824
Whole cell extracts (15 µg total protein per lane) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with the 1 µg/mL of Purified anti-MAVS (clone W21144C) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405) at a 1:5000 dilution. Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control at a 1:25000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • W21144C_PURE_MAVS_Antibody_1_010824
    Whole cell extracts (15 µg total protein per lane) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with the 1 µg/mL of Purified anti-MAVS (clone W21144C) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405) at a 1:5000 dilution. Direct-Blot™ HRP anti-β-actin (Cat. No. 664804) was used as a loading control at a 1:25000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • W21144C_PURE_MAVS_Antibody_2_010824
    MCF7 cells were fixed with ice-cold methanol and blocked with 5% FBS for 1 hour at room temperature. Cells were then intracellularly stained with 5 µg/mL of Purified anti-MAVS (clone W21144C) (green, panel A) and anti-HSP60 (Cat. No. 681502), followed by incubation with 2.5 µg/mL of Alexa Fluor® 488 Goat anti-rat IgG (Cat. No. 405418) and Alexa Fluor® 594 Goat anti-mouse IgG (red, panel B) (Cat. No. 405326) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801) (blue, panel C). The image was captured with a 40X objective. Scale bar: 25 µm
  • W21144C_PURE_MAVS_Antibody_3_010824
    MCF7 cells (mock) (panels A and B) and MCF knockdown cells (panels C and D) were fixed with ice-cold methanol and blocked with 5% FBS for 1 hour at room temperature. Cells were then intracellularly stained with 5 µg/mL of Purified anti-MAVS (clone W21144C), followed by incubation with 2.5 µg/mL of Alexa Fluor® 594 Goat anti-rat IgG (Cat. No. 405422) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801) (blue, panels B and D). The image was captured with a 40X objective. Scale bar: 25 µm
  • W21144C_PURE_MAVS_Antibody_4_010824
    IHC staining using Purified anti-MAVS (clone W21144C) on paraffin-embedded human testis (panel A) and human colon (panel B). Following antigen retrieval using 1X Citrate buffer (Cat. No. 420901), the tissue was incubated with 1 µg/mL of Purified anti-MAVS (clone W21144C) overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 647 goat anti-rat IgG (Cat. No. 405416) for one hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801) (blue). The image was captured with a 40x objective. Scale bar: 25 µm
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624351 25 µg £107
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624352 100 µg £271
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Description

Mitochondrial antiviral signaling protein (MAVS) is essential and specific for innate immunity by activating IRF3, IRF7 and NF-κB in response to viral infection. MAVS is located on the outer membranes of mitochondria, peroxisomes, and mitochondria-associated endoplasmic reticulum membranes (MAM). The MAVS protein contains an N-terminal CARD domain and a C-terminal mitochondrial transmembrane domain. MAVS acts downstream of the RIG-I RNA helicase and sensing viral RNA, leading to the recruitment of IKKε, TRIF and TRAF6. Some viruses have developed strategies to evade these innate defense mechanisms by using proteases that cleave MAVS and prevent its localization to mitochondria. The C-terminal region of MAVS cleavage by the hepatitis C virus (HCV) protease allows HCV to evade the host immune system.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial Recombinant human MAVS protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 0.5 - 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The clone does not cross-react with mouse in western blotting (WB).

For immunocytochemistry (ICC), we recommend to use 4% PFA fixation followed by permeabilization with 0.5% Triton X-100 or ice cold-methanol, or ice-cold methanol fixation.

For immunohistochemistry (IHC-P), antigen retrieval with Citrate Buffer (Cat. No. 420901) or Tris-EDTA pH 9.0 is recommended.

RRID
AB_3097498 (BioLegend Cat. No. 624351)
AB_3097498 (BioLegend Cat. No. 624352)

Antigen Details

Structure
MAVS protein is a 540 amino acid protein with a predicted molecular weight of 57 kD
Distribution

Ubiquitously expressed, with highest levels in heart, skeletal muscle, liver, placenta and peripheral blood leukocytes

Function
Innate immunity
Interaction
RIGI, IFIH1/MDA5, TRAF2, TRAF6, C1QBP, FADD, RIPK1, CHUK, IKBKB and NLRX1
Cell Type
Epithelial cells, Leukocytes, Monocytes, T cells
Biology Area
Cell Biology, Immunology, Innate Immunity
Molecular Family
Innate Immune Signaling
Antigen References
  1. Seth RB, et al. 2005. Cell. 122:669-82.
  2. Sun Q, et al. 2006. Immunity. 24:633-42.
  3. Xu LG, et al. 2005. Mol Cell. 19: 727-40.
  4. Yoneyama M, et al. 2004. Nat Immunol. 5:730-7.
  5. Lin R, et al. 2006. J Virol. 80:6072-83.
  6. Chen Z, et al. 2007. J Virol. 81:964-76.
  7. Li XD, et al. 2005. PNAS. 102:17717-22.
Gene ID
57506 View all products for this Gene ID
UniProt
View information about MAVS on UniProt.org
Go To Top Version: 1    Revision Date: 01/08/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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