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Purified anti-Mcl-1 Antibody

Pricing & Availability
Clone
W16014A (See other available formats)
Regulatory Status
RUO
Other Names
Induced myeloid leukemia cell differentiation protein Mcl-1, Bcl-2-like protein 3 (BCL2-L-3), Bcl-2-related protein EAT/mcl1,
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W16014A_PURE_Mcl-1_Antibody_032317
Total cell lysates (15µg protein) from Raji, MCF-7 and Raw 264.7 were resolved by 4-20% Tris-glycine gel electrophoresis, transferred to nitrocellulose, and probed with 0.2, 0.5 and 2µg/mL purified anti-MCL-1 antibody (clone W16014A ) antibody (upper). Proteins were visualized using a goat anti-rat-IgG secondary antibody conjugated to HRP and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin Antibody was used as a loading control (lower). Lane M: Molecular Weight ladder.
  • W16014A_PURE_Mcl-1_Antibody_032317
    Total cell lysates (15µg protein) from Raji, MCF-7 and Raw 264.7 were resolved by 4-20% Tris-glycine gel electrophoresis, transferred to nitrocellulose, and probed with 0.2, 0.5 and 2µg/mL purified anti-MCL-1 antibody (clone W16014A ) antibody (upper). Proteins were visualized using a goat anti-rat-IgG secondary antibody conjugated to HRP and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin Antibody was used as a loading control (lower). Lane M: Molecular Weight ladder.
  • W16014A_PURE_Mcl-1_Antibody_IF_010818
    Raw264.7 cells were fixed with 4% paraformaldehyde (PFA) for fifteen minutes, permeabilized with 0.5% Triton X-100 for three minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with 0.0 and 2.0 µg/mL anti-MCL-1 antibody (clone W16014A ) overnight at 4°C followed by Alexa Fluor® 594 (red) conjugated goat anti-rat IgG for one hour at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
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695702 100 µg £189
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Description

Myeloid cell leukemia sequence 1 (MCL-1), also known as Bcl2-L-3, is a member of the pro-survival BCL-2 family of apoptotic regulatory proteins. MCL-1 plays a crucial role in the survival and development of various cell types by binding to proapoptotic proteins and inhibiting the mitochondrial apoptotic pathway. Unlike Bcl-2 and Bcl-xL, MCL-1 lacks a BH4 domain and contains an N-terminal PEST region with specific sequences for ubiquitination, phosphorylation, and caspase cleavage sites, which are important in regulating the half-life of MCL-1. Phosphorylation of at T92 and T163 by ERK inhibits MCL-1 turnover, whereas phosphorylation by GSK3β at S159 facilitates proteosomal degradation. Increased expression of MCL-1 is correlated with tumor survival and is associated with resistance of chemotherapeutics.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Full length mouse MCL-1 recombinant protein expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified 
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 2.0 µg per ml. For immunocytochemistry, a concentration range of 0.5 - 2.5 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone W16014A does not cross-react with human (in-house tested). This antibody recognizes the two reported isoforms of MCL-1 (MWs: 31 and 35 KDa).

RRID
AB_2650880 (BioLegend Cat. No. 695702)

Antigen Details

Structure
There are two reported isoforms of MCL-1 (MWs: 31 and 35 kDa). The protein contains PEST, BH1, BH2, and BH3 domains.
Distribution

Nucleus, Cytoplasm, Mitochondria.

Function
MCL-1 is a prosurvival protein belonging to the BCL-2 family and is required for the survival and development of various cell types.
Interaction
MCL-1 forms a homodimer. Interacts with HIF3A, PMAIP1, BBC3, BAX, BAK1, TPT1, BOP, BCL2L11, BAD, BOK, BIK, and BMF.
Cell Type
Tregs
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology
Antigen References

1. Kozopas K, et al. 1993. P. Natl. Acad. Sci. USA 90:3516.
2. Zhou P, et al. 1997. Blood 89:630.
3. Chao J, et al. 1998. Mol. Cell. Biol. 18:4883.
4. Epling-Burnette P, et al. 2001. J. Immunol. 166:7486.
5. Moulding D, et al. 1998. Blood 92:2495.
6. Inoshita S, et al. 2002. J. Biol. Chem. 277(46):43740.
7. Opferman JT, et al. 2003. Nature. 426:671.

Gene ID
4170 View all products for this Gene ID
UniProt
View information about Mcl-1 on UniProt.org
Go To Top Version: 1    Revision Date: 03/24/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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