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Purified anti-MINA Antibody

Pricing & Availability
Clone
1H6B44 (See other available formats)
Regulatory Status
RUO
Other Names
Bifunctional lysine-specific demethylase and histidyl-hydroxylase MINA, 60S ribosomal protein L27a histidine hydroxylase, Histone lysine demethylase MINA, MYC-induced nuclear antigen, Nucleolar protein 52 (NO52), Ribosomal oxygenase MINA (ROX), MDIG
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
1H6B44_Pure_MINA_Antibody_1_WB_01282016
Total cell lysates (15 µg total protein) from Daudi, RPMI8226, A431, U87-MG, SH-SY5Y, and THP-1 (reduced expression control) cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-MINA Antibody, clone 1H6B44, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin Antibody (Cat. No. 643807) was used as a loading control at a 1:5000 dilution (lower). Lane M: Molecular Weight marker. Predicted expression data was obtained from Human Protein Atlas.
  • 1H6B44_Pure_MINA_Antibody_1_WB_01282016
    Total cell lysates (15 µg total protein) from Daudi, RPMI8226, A431, U87-MG, SH-SY5Y, and THP-1 (reduced expression control) cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-MINA Antibody, clone 1H6B44, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin Antibody (Cat. No. 643807) was used as a loading control at a 1:5000 dilution (lower). Lane M: Molecular Weight marker. Predicted expression data was obtained from Human Protein Atlas.
  • 1H6B44_Pure_MINA_Antibody_2_IF_01282016
    HeLa cells were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for three minutes, and blocked with 1% BSA for 60 minutes. Then the cells were intracellularly stained with 2.5 µg/ml purified anti-MINA (clone 1H6B44) antibody for one hour, followed by staining with Alexa Fluor® 594 conjugated goat anti-rat IgG antibody (red) and Alexa Fluor® 488 anti-Tubulin-α antibody (green) for one hour at room temperature. The image was captured with a 40X objective.
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683302 100 µg £182
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Description

MINA, also known as Mineral dust-induced gene (MDIG), is a member of the JmjC family of proteins and can act as a histone lysine demethylase and a ribosomal histidine hydroxylase. MINA is implicated in the demethylation of K9 trimethylated histone H3 (H3K9me3), regulating cell proliferation and survival. MINA has been suggested to be a novel oncogene associated with a variety of cancers. Elevated expression of MINA in human tumors has been linked to malignancy and poor prognosis. MINA has been identified as a transcriptional co-repressor of IL-4 gene and participates in the regulation of Th2 differentiation and Th2 bias. MINA appears to be a regulator of the Treg/Th17 balance by promoting differentiation of Th-17 cells and inhibiting FOXP3 expression.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant protein (300-465 aa) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg/ml. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2566795 (BioLegend Cat. No. 683302)

Antigen Details

Structure
465 amino acids with a predicted molecular weight of 52.8 kD. Contains a JmjC domain responsible for lysine demethylation of histones.
Distribution

Nucleus and nucleolus.

Function
MINA is an oxygenase exerting histone lysine demethylase and ribosomal histidine hydroxylase activities. It is involved in cell proliferation, survival, and T cell differentiation.
Interaction
MINA interacts with Srp72, NOP56, RPL10, EIF3E, HNRNPU, RNPS1, RPL35, and RRBP1.
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Thakur C, et al. 2015. Genes Cancer 6:288.
2. Pillai MR, et al. 2014. Curr. Opin. Immunol. 31:38.
3. Lian S, et al. 2013. PLoS One 8:e80638.
4. Hemmers S, et al. 2009. Nat. Immunol. 10:806.
5. Lu Y, et al. 2009. Cell Cycle. 8:2101.
6. Tsuneoka M, et al. 2004. Clin. Cancer Res. 10:7347.

Gene ID
84864 View all products for this Gene ID
UniProt
View information about MINA on UniProt.org
Go To Top Version: 2    Revision Date: 12/07/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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