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Purified anti-MLKL Antibody

Pricing & Availability
Clone
W17104A (See other available formats)
Regulatory Status
RUO
Other Names
MLKL, HMLKL
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W17104A_Purified_MLKL_Antibody_040219
Western blot analysis of 15µg cell lysates from human PC-3, HaCaT, HeLa (Positive), Daudi (Negative) and Raw264.7 (Mouse) cells. Samples were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, probed with 1 µg/mL anti-MLKL antibody, clone W17104A, followed by incubation with horseradish peroxidase (HRP) labeled goat anti-rat secondary antibody (Cat. No. 405405, 1:3000 dilution). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904, 1:10000 dilution). Western-Ready™ ECL Substrate Kit (Cat. No. 426303) was used for developing.
  • W17104A_Purified_MLKL_Antibody_040219
    Western blot analysis of 15µg cell lysates from human PC-3, HaCaT, HeLa (Positive), Daudi (Negative) and Raw264.7 (Mouse) cells. Samples were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, probed with 1 µg/mL anti-MLKL antibody, clone W17104A, followed by incubation with horseradish peroxidase (HRP) labeled goat anti-rat secondary antibody (Cat. No. 405405, 1:3000 dilution). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904, 1:10000 dilution). Western-Ready™ ECL Substrate Kit (Cat. No. 426303) was used for developing.
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666801 25 µg £70
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666802 100 µg £174
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Description

MLKL has a four-helix bundle (4HB) domain at the N-terminal and a C-terminal PsKD connected via a two-helix linker. MLKL is important in the necroptotic signaling pathways. During the process, phosphorylated RIP3 (receptor-interacting protein 3) is recruited and phosphorylates MLKL which can induce the oligomerization of MLKL. Oligomerized MLKL translates to the plasma membrane, destroying the membrane integrity and inducing necroptosis. Knockdown or inhibition of MLKL prohibits necrosis. High expression of MLKL and RIP3 are reported in inflammatory bowel disease. MLKL is also involved in endosomal trafficking and extracellular vesical generation, which is necroptosis independent.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human recombinant MLKL Protein (1-193 a.a.) expressed in E. Coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 2.5 µg per ml (1:200 - 1:2000 dilution). It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2801124 (BioLegend Cat. No. 666801)
AB_2801124 (BioLegend Cat. No. 666802)

Antigen Details

Distribution

Cytoplasm

Function
Necroptosis
Interaction
RIP3
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Death, Signal Transduction
Molecular Family
Protein Kinases/Phosphatase
Gene ID
197259 View all products for this Gene ID
UniProt
View information about MLKL on UniProt.org
Go To Top Version: 1    Revision Date: 04/02/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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