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Purified anti-MMP-9 Antibody (Previously Covance catalog# MMS-5163)

Pricing & Availability
Clone
L51/82 (See other available formats)
Regulatory Status
RUO
Other Names
GELB, MMP9, gelatinase B, 92 kD gelatinase, 92 kD type IV collagenase, 92-kD type IV collagenase, matrix metalloproteinase 9 (gelatinase B 92-kD type IV collagenase), matrix metalloproteinase 9 (gelatinase B, 92-kD type IV collagenase)
Previously
Covance Catalog# MMS-5163
Isotype
Mouse IgG2a
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Product Citations
publications
L51slash82_Purified_MMP-9_Antibody_1_040618
IHC staining of purified anti-MMP-9 antibody (clone L51/82) on formalin-fixed paraffin-embedded human spleen tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/mL of the primary antibody overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 10X objective. Scale bar: 50 µm
  • L51slash82_Purified_MMP-9_Antibody_1_040618
    IHC staining of purified anti-MMP-9 antibody (clone L51/82) on formalin-fixed paraffin-embedded human spleen tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/mL of the primary antibody overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 10X objective. Scale bar: 50 µm
  • L51slash82_Purified_MMP-9_Antibody_2_040618
    IHC staining of purified anti-MMP-9 antibody (clone L51/82) on formalin-fixed paraffin-embedded human spleen tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/mL of the primary antibody overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • L51slash82_Purified_MMP-9_Antibody_3_040618
    Western blot of purified anti-MMP-9 antibody (clone L51/82). Lanes 1: Molecular weight marker; Lanes 2:20 µg of human spleen lysate; Lanes 3:20 µg of rat spleen lysate. The blots were incubated with 5 µg/mL of clone L51/82 or mouse IgG2a overnight at 4°C, followed by incubation with HRP goat anti-mouse IgG antibody (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • L51slash82_Purified_MMP-9_Antibody_4_040618
    Western blot of purified anti-MMP-9 antibody (clone L51/82). Lanes 1: Molecular weight marker; Lanes 2:20 µg of mouse spleen lysate. The blots were incubated with 1 µg/mL of clone L51/82 or mouse IgG2a overnight at 4°C, followed by incubation with HRP goat anti-mouse IgG antibody (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
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819701 100 µL £113
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Description

Matrix metalloproteinases (MMPs) are zinc-dependent endopeptidases that degrade substances within the extracellular matrix. The MMP family includes different groups of enzymes, such as, collagenases, gelatinases, stromelysins, transmembrane MMPs, matrilysins and others. MMPs are secreted as proenzymes that have to be cleaved in order to be activated. Other MMPs, plasmins as well as other factors activate MMPs. MMPs are thought to play an important role in tissue remodeling associated with various physiological and pathological processes. MMP9 degrades proteins in the extracellular matrix and activates growth factors like proTGFb and proTNFa. MMP-9 contributes to the invasion and metastasis of various human malignancies. Clone F11P2C3 has been shown to be useful for western blotting and immunohistochemistry of human MMP9.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a full-length fusion protein corresponding to amino acids 1-708 of rat MMP9 protein.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application

IHC-P - Quality tested
WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. For western blotting, the suggested use of this reagent is 1.0 - 10.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Kuo PC, et al. 2021. Brain Commun. 3:fcab187. PubMed
  2. Lee JH, et al. 2022. EBioMedicine. 77:103903. PubMed
  3. Velasquez M, et al. 2023. Antibodies (Basel). 12:. PubMed
  4. Ito H, et al. 2021. Neuropsychopharmacology. 46:442. PubMed
  5. Swieboda D, et al. 2020. Front Immunol. 1.697916667. PubMed
  6. Brezovakova V, et al. 2022. Cells. 11:. PubMed
RRID
AB_2564833 (BioLegend Cat. No. 819701)

Antigen Details

Structure
Expected MW: 82 and 92 kD
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Neuroinflammation, Neuroscience
Molecular Family
Enzymes and Regulators
Gene ID
81687 View all products for this Gene ID
UniProt
View information about MMP-9 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 07/09/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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