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Purified anti-MMP3 Antibody

Pricing & Availability
Clone
M4405F10 (See other available formats)
Regulatory Status
RUO
Other Names
Progelatinase, STR1, Transin, Stromelysin I, MMP-3, Transin-1, STMY1, SL-1, CHDS6
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
M4405F10_PURE_MMP3_Antibody_WB_082115
Recombinant human MMP3 (50 ng) treated with (lane 2) and without (lane 1) 1 mM of p-aminophenylmercuric acetate (APMA) and recombinant mouse MMP3 (50 ng, lane 3) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-MMP3 (clone M4405F10) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection.
  • M4405F10_PURE_MMP3_Antibody_WB_082115
    Recombinant human MMP3 (50 ng) treated with (lane 2) and without (lane 1) 1 mM of p-aminophenylmercuric acetate (APMA) and recombinant mouse MMP3 (50 ng, lane 3) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-MMP3 (clone M4405F10) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection.
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679202 100 µg £157
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Description

MMP3 is a member of the matrix metalloproteinase family proteins (MMPs). Members of this family are structurally related to zinc-containing enzymes that degrade the extracellular matrix (ECM), connective tissue proteins in normal physiological processes such as embryonic development, reproduction, and tissue remodeling, and in disease processes such as arthritis and metastasis as well. MMP3 consists of a prodomain that is cleaved upon activation, a catalytic domain containing the zinc binding site, a short hinge region, and a carboxyl terminal (hemopexin-like repeats) domain. Substrates of MMP3 include collagen α chains, aggrecan, laminin, fibronectin, elastin, casein, α1-antitrypsin, myelin basic protein, IL1β, IGFBP3, pro MMP1, pro MMP7, pro MMP8, pro MMP9, and pro MMP13. MMP3 is thought to be involved in wound repair, progression of atherosclerosis, and tumor initiation. Also, it has been known that a polymorphism in the MMP3 promoter region is associated with the risk of coronary heart disease and myocardial infarction due to lower MMP3 proteolytic activity and higher ECM deposition in atherosclerotic lesions.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human MMP-3, amino acids Tyr18 -Cys477, was expressed with N-terminal His9-SGGGSGGGIEGR tag in 293E cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Kuo PC, et al. 2021. Brain Commun. 3:fcab187. PubMed
RRID
AB_2566077 (BioLegend Cat. No. 679202)

Antigen Details

Structure
Belongs to the peptidase M10A family. Monomer. Contains 4 hemopexin-like domains with an approximate predicted molecular weight of 54 kD. Mature form of MMP3 upon activation by p-aminophenylmercuric acetate (APMA), has predicted molecular weight of 45 kD.
Distribution
Extracellular. MMP3 is expressed by fibroblasts, chondrocytes, osteoblasts, endothelial cells, smooth muscle cells, and macrophages.
Function
MMP3 degrades fibronectin, laminin, gelatins of type I, III, IV, and V, collagens III, IV, X, and IX, and cartilage proteoglycans. It activates procollagenases. MMP3 is thought to play a prominent role in the pathogenesis of rheumatoid arthritis.
Interaction
Fibronectin, laminin, collagens III, IV, IX, and X, cartilage proteoglycans, decorin, osteopontin, and IBP-3.
Ligand/Receptor
Tissue inhibitors of metalloproteinases (TIMPs).
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Neuroinflammation, Neuroscience
Molecular Family
Enzymes and Regulators
Antigen References

1. Matrisian LM. 1990. Trends Genet. 4:121.
2. Nagase H and Woessner JF Jr. 1999. J. Biol. Chem. 274:21491.
3. Humphries SE, et al. 1998. Atherosclerosis 139:49.
4. Kader AK, et al. 2007. Clin. Cancer Res. 13:2614.
5. Gill SE and Parks WC. 2008. Int. J. Biochem. Cell Biol. 40:1334.
6. Mazor R, et al. 2013. J. Biol. Chem. 288:598.
7. Herath TD, et al. 2013. BMC Microbiol. 13:73.

Gene ID
4314 View all products for this Gene ID
UniProt
View information about MMP-3 on UniProt.org
Go To Top Version: 1    Revision Date: 08/21/2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

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