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Purified anti-mouse AhR Antibody

Pricing & Availability
Clone
W16012C (See other available formats)
Regulatory Status
RUO
Other Names
bHLHe76, aryl hydrocarbon receptor, Aryl hydrocarbon receptor, RP85
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W16012C_PURE_AhR_Antibody_042821.png
C57BL/6 mouse splenocytes were cultured for 3 days with (filled histogram) or without (dashed histogram) recombinant human TGF-β1 and recombinant mouse IL-6. Cells were removed from culture media, washed, and finally fixed and permeabilized. Cells were then intracellularly stained with purified anti-mouse AhR (clone W16012C) followed by anti-rat IgG PE.
  • W16012C_PURE_AhR_Antibody_042821.png
    C57BL/6 mouse splenocytes were cultured for 3 days with (filled histogram) or without (dashed histogram) recombinant human TGF-β1 and recombinant mouse IL-6. Cells were removed from culture media, washed, and finally fixed and permeabilized. Cells were then intracellularly stained with purified anti-mouse AhR (clone W16012C) followed by anti-rat IgG PE.
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162702 100 µg £189
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Description

Aryl Hydrocarbon Receptor or AhR is a bHLH transcription factor that is activated by ligands such as TCDD. It is expressed in many immune cells and perform an important role in immune regulation. AhR is induced under TH17-polarizing conditions. In its inactive state, AhR presents in the cytoplasm, and upon ligand binding it translocates to the nucleus. Activation of AhR is tightly controlled by Ubiquitination, ligand metabolism, and complex disruption.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2894627 (BioLegend Cat. No. 162702)
Go To Top Version: 1    Revision Date: 04/28/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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