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Purified anti-mouse Aiolos Antibody

Pricing & Availability
Clone
8B2 (See other available formats)
Regulatory Status
RUO
Other Names
IKAROS family zinc finger 3 (Ikzf3), zinc finger protein Aiolos, Zfpn1a3, Znfn1a3
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
8B2_Purified_Aiolos_Antibody_WB_050718
Total lysates (15 µg protein) from Jurkat (Lane 1), EL4 (Lane 2), and A20 (Lane 3) cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 2 µg/mL Purified anti-Aiolos Antibody, clone 8B2 (upper). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for the anti-Aiolos Antibody or 1:5000 diluted Direct-Blot™ HRP anti-β-Actin Antibody, clone 2F1-1(lower). Lane M: Molecular weight ladder
  • 8B2_Purified_Aiolos_Antibody_WB_050718
    Total lysates (15 µg protein) from Jurkat (Lane 1), EL4 (Lane 2), and A20 (Lane 3) cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 2 µg/mL Purified anti-Aiolos Antibody, clone 8B2 (upper). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for the anti-Aiolos Antibody or 1:5000 diluted Direct-Blot™ HRP anti-β-Actin Antibody, clone 2F1-1(lower). Lane M: Molecular weight ladder
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653201 25 µg £97
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653202 100 µg £238
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Description

Aiolos, an Ikaros family member, is also known as Izkf3. It has a molecular weight of 58 kD and shows 70% homology to the Ikaros isoform, Ik-1, particularly in the DNA binding and C-terminal domains. Aiolos can form a heteromer with Ikaros or exist as a homodimer. The homomeric complex is a potent transcriptional activator.  Unlike Ikaros, Aiolos is expressed in late fetal thymus and adult lymphoid organs. Among the adult hematopoietic progenitors, Aiolos is expressed at low levels in mulitpotent but not in pluripotent stem cells. When these multipotent progenitors become more restricted to T and B lymphoid pathways there is an upregulation in Aiolos expression. The highest expression of Aiolos is detected in the spleen (especially in B-cells) followed by thymus and bone marrow, and cannot be detected in the non-lymphoid organs. Aiolos plays an important role in lymphocyte proliferation and differentiation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
N-terminal half of mouse Aiolos fused to GST
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Previous lots of this product may have been formulated with 0.1% or 0.05% NaN3 instead of 0.09% NaN3. For further information please contact BioLegend Technical Support or Customer Service.
Concentration
0.5 mg/mL
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICFC, IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 0.5 µg/ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: intracellular flow cytometry1, immunoprecipitation1, and Western blotting2.

Does not cross-react with human.

View more applications data for a different format of this clone in our Scientific Poster Library.

This product is for research use only, cannot be used on or in humans, and is not to be used for commercial purposes.  Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited.  In order to obtain a license to use this product for commercial purposes, contact the Regents of the University of California.

Application References

(PubMed link indicates BioLegend citation)
  1. Laszlo G, et al. 1993. J. Immunol. 150:5252. (ICFC, IP)
  2. Thompson EC, et al. 2007. Immunity 26:335. (WB)
Product Citations
  1. Wei SC, et al. 2019. Immunity. 50:1084. PubMed
RRID
AB_2561709 (BioLegend Cat. No. 653201)
AB_2561709 (BioLegend Cat. No. 653202)

Antigen Details

Structure
509 amino acids with predicted MW of 58 kD
Distribution

Nucleus, cytoplasm
Restricted to lymphoid lineage, expressed in B, NK and T cells

Function
Transcription factor that plays an important role in the regulation of lymphocyte differentiation; plays an essential role in regulation of B-cell differentiation, proliferation, and maturation to an effector state
Interaction
Homodimer and heterodimer with other IKAROS family members; interacts with IKZF4, IKZF5, IKZF1, HRAS, FOXP3, and BCL21L isoform Bcl-X(L); associates with histone deacetylase complexes containing HDAC1, MTA2 and SIN3A
Cell Type
B cells, NK cells, T cells
Biology Area
Cell Biology, Chromatin Remodeling/Epigenetics, Immunology, Innate Immunity, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Morgan B, et al. 1997. EMBO J. 16:2004.
2. Wang JH, et al. 1998. Immunity 9:543.
3. Cariappa A, et al. 2001. Immunity 14:603.

Gene ID
22780 View all products for this Gene ID
UniProt
View information about Aiolos on UniProt.org
Go To Top Version: 4    Revision Date: 02/08/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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