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Purified anti-mouse Arginase 1 Antibody

Pricing & Availability
Clone
W21047I (See other available formats)
Regulatory Status
RUO
Other Names
Arginase 1, liver type Arginase, Type I Arginase, ARG1
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W21047I_-Arginase-1_Purified_FC_061234
C57BL/6 mice bone marrow derived macrophages were polarized to M2a for 48 hours with IL-4. Then cells were fixed and permeabilized with Cyto-Fast™ Fix/Perm Buffer Set and intracellularly stained with purified anti-mouse Arginase 1 (clone W21047I) (left), or purified rat IgG2b, κ isotype control (right), followed by anti-rat IgG PE. Then, cells were costained with anti-mouse F4/80 APC.
  • W21047I_-Arginase-1_Purified_FC_061234
    C57BL/6 mice bone marrow derived macrophages were polarized to M2a for 48 hours with IL-4. Then cells were fixed and permeabilized with Cyto-Fast™ Fix/Perm Buffer Set and intracellularly stained with purified anti-mouse Arginase 1 (clone W21047I) (left), or purified rat IgG2b, κ isotype control (right), followed by anti-rat IgG PE. Then, cells were costained with anti-mouse F4/80 APC.
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165802 100 µg £238
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Description

ARG1 (Arginase 1), is a marker for the M2 anti-inflammatory macrophage subset that hydrolyzes l-arginine into urea and ornithine, and plays an important role in wound healing, antiviral immune responses, and atherosclerosis. The inherited deficiency of this enzyme results in argininemia, which is an autosomal recessive disorder characterized by hyperammonemia. Recent studies show that intracellular pathogens induce the expression of ARG1 in mouse macrophages through the Toll-like receptor (TLR) pathway. The deletion of this enzyme in macrophages favors host survival. Other studies indicate that ARG1 also plays a role in T cell responses and selective antagonists of ARG1 might prove beneficial in fighting diseases in which T-cell responses are inappropriately suppressed.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
E. coli expressed protein aa 1-323
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone W21047I does not block the staining of reference clone A1eXF5 on the target cells (BMDM). 

RRID
AB_3068107 (BioLegend Cat. No. 165802)

Antigen Details

Structure
322 amino acids with a predicted molecular weight of approximately 35 kD
Distribution

Express on dendritic cells & macrophages. Expressed predominantly in the liver. It can also be induced in macrophages. It is cytoplasmic target.

Function
Arginase 1 plays an important role in processes such as wound healing, antiviral immune response, and atherosclerosis.
Interaction
Binds 2 manganese ions per subunit as a cofactor
Ligand/Receptor
l-arginine
Cell Type
Neutrophils
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Enzymes and Regulators
Antigen References
  1. Wu G, et al. 1998. Biochem J. 336:1.
  2. El Kasmi KC, et al. 2008. Nat Immunol. 9:1399-406.
  3. Pesce JT, et al. 2009. PLoS Pathog. 5:e1000371.
  4. Burrack KS, et al. 2014. Front Immunol. 5:428.
  5. Pourcet B, et al. 2013. Trends Cardiovasc Med. 23:143-52.
  6. Bronte V, et al. 2003. Trends Immunol. 24:302-6.
Gene ID
383 View all products for this Gene ID
UniProt
View information about Arginase 1 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 06/12/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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