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Purified anti-mouse CCL22 (MDC) Antibody

Pricing & Availability
Clone
W20219K (See other available formats)
Regulatory Status
RUO
Other Names
Macrophage derived cytokine (MDC), Small inducible cytokine subfamily A, member 22 (Scya22), stimulated T cell chemotactic protein (STCP-1), activated B cell-derived chemokine-1 (ABCD-1) DC, B cell-derived chemokine (DC/B-CK)
Isotype
Rat IgG2b, κ
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Product Citations
publications
W20219K_Pure_CCL22_Antibody_1_110824
GM-CSF generated bone marrow-derived dendritic cells (BMDCs) were treated with 1X Monensin (Cat. No. 420701) for 16-24 hours. Cells were then fixed and permeabilized with Cyto-Fast™ Fix/Perm Buffer (Cat. 426803) and intracellularly stained with purified anti-mouse CCL22 (MDC) (clone W20219K) (left) or purified rat IgG2b, κ isotype control (right) followed by anti-rat IgG PE. Cells were then stained with anti-mouse/human CD11b (clone M1/70) APC.
  • W20219K_Pure_CCL22_Antibody_1_110824
    GM-CSF generated bone marrow-derived dendritic cells (BMDCs) were treated with 1X Monensin (Cat. No. 420701) for 16-24 hours. Cells were then fixed and permeabilized with Cyto-Fast™ Fix/Perm Buffer (Cat. 426803) and intracellularly stained with purified anti-mouse CCL22 (MDC) (clone W20219K) (left) or purified rat IgG2b, κ isotype control (right) followed by anti-rat IgG PE. Cells were then stained with anti-mouse/human CD11b (clone M1/70) APC.
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167502 100 µg £274
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Description

CCL22, also known as macrophage-derived chemokine (MDC), is a conventional CC chemokine that is expressed constitutively under homeostatic conditions and can be induced in several cell types upon activation.

Mouse CCL22 is expressed at high levels in dendritic cells and activated B lymphocytes. Low levels of mouse MDC mRNA is also detectable in lung, unstimulated spleen cells, lymph node cells, and in thymocytes. Constitutive expression of CCL22 is mediated by T cell–derived GM-CSF.

The only known receptor for CCL22 is CCR4, which is mainly expressed by activated Th2-biased CD4+ T cells and by regulatory T cells. CCL22 displays chemotactic activity for natural killer cells, chronically activated T lymphocytes, monocytes, and dendritic cells, and may play a role in recruiting activated T lymphocytes to inflammatory sites and other aspects of activated T lymphocyte physiology.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse CCL22 (aa25-92)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our Cyto-Fast™ Fix/Perm Buffer Set. For flow cytometric staining, the suggested use of this reagent is  ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance in each application.

Antigen Details

Structure
Chemokine
Distribution

Macrophages, NK cells, thymic epithelial cells and intestinal epithelial cells

Function
CCL22 induces the migration of CCR4 + Th2 cells, Tregs, DC, and NK cells. It is induced by LPS and CD40 antibody in macrophages. It is upregulated by Th2 cytokines (IL-4 and IL-5) and downregulated by Th1 cytokines (IFNγ).
Interaction
Dendritic cells, NK cells, Th2 cells, T regs, CLA+ skin-homing memory/effector T cells
Ligand/Receptor
CCR4
Biology Area
Cell Biology, Signal Transduction
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Kanazawa N, et al. 1999. Eur. J. Immunol. 29: 1925–32
  2. Schaniel C, et al. 1998. J. Exp. Med. 188:451.
  3. Andrew DP, et al. 1998. J. Immunol. 161(9):5027-38.
  4. Ghadially H, et al. 2005. J Immunol. 174(9):5620–29
  5. Godiska R, et al. 1997. J. Exp. Med. 185:1595.
  6. Chang, M-S. et al. 1997. J. Biol. Chem. 272:25229
  7. Piseddu I, et al. 2020. J Immunol. 205(8): 2056–65.
Gene ID
20299 View all products for this Gene ID
UniProt
View information about CCL22 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 11/08/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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