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Purified anti-mouse CCL24 Antibody

Pricing & Availability
Clone
A18110F (See other available formats)
Regulatory Status
RUO
Other Names
MPIF-2
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A18110F_PURE_CCL24_Antibody_041023
Direct ELISA showing purified anti-mouse CCL24 (clone A18110F) bound to plate-immobilized recombinant mouse CCL24 (Eotaxin-2) protein (Cat. No. 585102). The wells were incubated with serially diluted primary antibody at room temperature for 2 hours in 1% BSA-PBS, followed by incubation with HRP Goat anti-rat IgG (Cat. No. 405405). TMB High Sensitivity Substrate Solution (Cat. No. 421501) was used for detection. Absorbance was measured at 450 nm.
  • A18110F_PURE_CCL24_Antibody_041023
    Direct ELISA showing purified anti-mouse CCL24 (clone A18110F) bound to plate-immobilized recombinant mouse CCL24 (Eotaxin-2) protein (Cat. No. 585102). The wells were incubated with serially diluted primary antibody at room temperature for 2 hours in 1% BSA-PBS, followed by incubation with HRP Goat anti-rat IgG (Cat. No. 405405). TMB High Sensitivity Substrate Solution (Cat. No. 421501) was used for detection. Absorbance was measured at 450 nm.
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612152 100 µg £161
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Description

CLL24 was initially cloned from a library of activated macrophages, and using human eosinophils, it was shown that it binds to the receptor of eotaxin-1 (CCL11) and MCP-4 (CCL13). In addition, CCL24 shares the CCR3 receptor with CCL26, CCL5, and CCL7. Eotaxin/CCR3 expression has been studied extensively in the pathogenesis of asthma and allergy. In addition, the eotaxin/CCR3 axis has been associated to additional inflammatory and autoimmune disorders including inflammatory bowel disease, multiple sclerosis, eosinophilic esophagitis, and rheumatoid arthritis. CCL24 has also been associated with neovascular age related macular degeneration.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CCL24
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

Direct ELISA - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Direct ELISA. The optimal concentration of this reagent for the specific application and the experiment design should be established by the end-user.

Additional Product Notes

This product is intended for sandwich-based immunoassay development on various assay platforms requiring antibody pairs. This antibody (clone A18110F) could function as a detection antibody when paired with purified anti-mouse CCL24 antibody (Cat. No. 612752) (clone A18110C) as the capture antibody and using Recombinant Mouse CCL24 (Eotaxin-2) protein (Cat. No. 585102) as the assay standard. For assay development flexibility purposes, this product is provided as a purified format.

RRID
AB_2941605 (BioLegend Cat. No. 612152)

Antigen Details

Structure
Chemokine
Distribution

CCL24 is expressed by epithelial cells, airway smooth muscle cells, and vascular endothelial cells. In addition, eotaxins are produced by cells isolated by bronchial alveolar lavage, including macrophages, monocytes, basophils, eosinophils, dendritic cells, and lymphocytes.

Function
CCL24 chemoattracts CCR3 bearing cells such as eosinophils, mast cells, basophils, and Th2 cells that play a role in allergic disorders. IL-4 and IL-13 induce CCL11 and CCL24 in esophagus and colonic epithelial cell, and IL-13 induces the production of CCL-24 in dendritic cells and alveolar macrophages. In addition, CCL24 is induced by IL-33 in lungs, and by IL-4 and LPS in monocytes and macrophages. The expression of CCL24 by alveolar epithelial cells requires histamine and its receptor H2R.
Interaction
Eosinophils, mast cells, Th2 lymphocytes, basophils, human dendritic cells, synovial tissue macrophages, lung epithelial cells, and airway smooth muscle cells.
Ligand/Receptor
CCR3
Biology Area
Cell Biology, Signal Transduction
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Patel VP, et al. 1997. J Exp Med. 185:1163-72.
  2. Beaulieu S, et al. 2002. J Immunol. 169:2925-36.
  3. Watanabe K, et al. 2002. J Immunol. 168:1911-8.
  4. Ablin JN, et al. 2010. Clin Exp Immunol. 161:276-83.
  5. Manousou P, et al. 2010. Clin Exp Immunol. 162:337-47.
  6. Stolarski B, et al. 2010. J Immunol. 185:3472-80.
  7. Crapster-Pregont M, et al. 2012. J Allergy Clin Immunol. 129:1621-7.
  8. Swartzendruber JA, et al. 2012. J Immunol. 188:536-40.
Gene ID
56221 View all products for this Gene ID
UniProt
View information about CCL24 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 04/10/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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