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Purified anti-mouse CD100 (SEMA4D) Antibody

Pricing & Availability
Clone
BMA-12 (See other available formats)
Regulatory Status
RUO
Other Names
Neuroimmune semaphorin 4D, Sema4D, M-Sema G, Semaphorin-C-like 2, Semaphorin-J, Semaj, Semcl2, Coll-4, Semacl2
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
BMA12_Purified_CD100_Antibody_FC_032514.jpg
C57BL/6 mouse splenocytes were stained with purified CD100/SEMA4D (clone BMA-12, filled histogram) or purified rat IgG2a, κ isotype control (open histogram), followed by anti-rat IgG PE.
  • BMA12_Purified_CD100_Antibody_FC_032514.jpg
    C57BL/6 mouse splenocytes were stained with purified CD100/SEMA4D (clone BMA-12, filled histogram) or purified rat IgG2a, κ isotype control (open histogram), followed by anti-rat IgG PE.
  • BMA12_purified_CD100_Antibody_IHCF_113016
    C57BL/6 mouse frozen kidney section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/mL of purified anti-mouse CD100 (clone BMA-12) and anti-mouse Podoplanin (clone 8.1.1) Alexa Fluor® 594 (green) overnight at 4°C, followed by 2.5 µg/mL of goat anti-rat IgG (clone Poly4054) Alexa Fluor® 647 (red) for two hours at room temperature. The image was captured with a 10X objective.
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147601 25 µg £70
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147602 100 µg £213
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Description

CD100, also known as Semaphorin-4D (SEMA4D), is a 150 kD, type I transmembrane protein, with one sema domain and one C2-type Ig domain. It is found as a homodimer on the cell surface. CD100 is expressed in the brain, kidney, heart, the majority of hematopoietic cells, and some malignant cells. CD100 is a potent inducer of angiogenesis and inhibitor of bone formation, and plays a role in lymphocyte activation, antibody production, dendritic cell maturation, and axon guidance. Its ligands are CD72 and Plexin-B1. CD100 is cleaved from the membrane of activated T and B cells, producing a soluble form.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CD100-Fc fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Verified
IP, ELISA - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 5.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications for the relevant formats include: immunoprecipitation from cell lysates2, capture antibody function in a sandwich ELISA assay2, and immunohistochemical staining of frozen brain sections4.

Application References

(PubMed link indicates BioLegend citation)
  1. Kumanogoh A, et al. 2000. Immunity. 13:621. (FC)
  2. Wang X, et al. 2001. Blood. 97:3498. (FC, IP, ELISA)
  3. Kumanogoh A, et al. 2002. J. Immunol. 169:1175. (FC)
  4. Moreau-Fauvarque C, et al. 2003 J. Neurosci. 23:9229. (IHC)
RRID
AB_2563413 (BioLegend Cat. No. 147601)
AB_2563413 (BioLegend Cat. No. 147602)

Antigen Details

Structure
Type I transmembrane protein, one sema domain, one C2-type immunoglobulin domain, homodimer, 150 kD.
Distribution

Highly expressed on most hematopoietic cells, is also found in the brain, kidney, and heart. Overexpressed by some malignant cell types.

Function
Potent inducer of angiogenesis, inhibitor of bone formation, plays a role in lymphocyte activation, antibody production and dendritic cell maturation. In neurons, regulates dendrite branching and axon guidance.
Ligand/Receptor
CD72, Plexin-B1.
Cell Type
B cells, Dendritic cells, Neutrophils, Tregs
Biology Area
Angiogenesis, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Neuroscience, Signal Transduction, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell. 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
20354 View all products for this Gene ID
UniProt
View information about CD100 on UniProt.org
Go To Top Version: 2    Revision Date: 11/30/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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