Purified anti-mouse CD100 SEMA4D Antibody anti-CD100

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Clone: BMA-12 (See other available formats)
Regulatory Status: RUO
Other Names: Neuroimmune semaphorin 4D, Sema4D, M-Sema G, Semaphorin-C-like 2, Semaphorin-J, Semaj, Semcl2, Coll-4, Semacl2
Isotype: Rat IgG2a, κ
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Product Citations: publications

C57BL/6 mouse splenocytes were stained with purified CD100/SEMA4D (clone BMA-12, filled histogram) or purified rat IgG2a, κ isotype control (open histogram), followed by anti-rat IgG PE.

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147601

25 µg

£70

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147602

100 µg

£213

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Description

CD100, also known as Semaphorin-4D (SEMA4D), is a 150 kD, type I transmembrane protein, with one sema domain and one C2-type Ig domain. It is found as a homodimer on the cell surface. CD100 is expressed in the brain, kidney, heart, the majority of hematopoietic cells, and some malignant cells. CD100 is a potent inducer of angiogenesis and inhibitor of bone formation, and plays a role in lymphocyte activation, antibody production, dendritic cell maturation, and axon guidance. Its ligands are CD72 and Plexin-B1. CD100 is cleaved from the membrane of activated T and B cells, producing a soluble form.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse CD100-Fc fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
IHC-F - Verified
IP, ELISA - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 5.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications for the relevant formats include: immunoprecipitation from cell lysates², capture antibody function in a sandwich ELISA assay², and immunohistochemical staining of frozen brain sections⁴.
RRID: AB_2563413 (BioLegend Cat. No. 147601)
AB_2563413 (BioLegend Cat. No. 147602)

Antigen Details

Structure: Type I transmembrane protein, one sema domain, one C2-type immunoglobulin domain, homodimer, 150 kD.
Distribution: Highly expressed on most hematopoietic cells, is also found in the brain, kidney, and heart. Overexpressed by some malignant cell types.
Function: Potent inducer of angiogenesis, inhibitor of bone formation, plays a role in lymphocyte activation, antibody production and dendritic cell maturation. In neurons, regulates dendrite branching and axon guidance.
Ligand/Receptor: CD72, Plexin-B1.
Cell Type: B cells, Dendritic cells, Neutrophils, Tregs
Biology Area: Angiogenesis, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Neuroscience, Signal Transduction, Synaptic Biology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell. 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.
Gene ID: 20354 View all products for this Gene ID
UniProt: View information about CD100 on UniProt.org

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Other Formats

View All CD100 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-mouse CD100 (SEMA4D)	BMA-12	FC,IHC-F,IP,ELISA
PE anti-mouse CD100 (SEMA4D)	BMA-12	FC
APC anti-mouse CD100 (SEMA4D)	BMA-12	FC

Certificate of Analysis

Go To Top Version: 2 Revision Date: 11/30/2016

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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