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Purified anti-mouse CD106 Antibody

Pricing & Availability
Clone
W21127K (See other available formats)
Regulatory Status
RUO
Other Names
VCAM-1, INCAM-110
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A. W21127K_PURE_CD106_FC_090723
C57BL/6 mouse bone marrow cells were stained with anti-mouse/human CD11b APC and purified anti-mouse CD106 (clone W21127K) (left) or purified rat IgG2a, κ isotype control (right) followed by PE goat anti-rat IgG2a.
  • A. W21127K_PURE_CD106_FC_090723
    C57BL/6 mouse bone marrow cells were stained with anti-mouse/human CD11b APC and purified anti-mouse CD106 (clone W21127K) (left) or purified rat IgG2a, κ isotype control (right) followed by PE goat anti-rat IgG2a.
  • B. W21127K_PURE_CD106_IHCP_092523
    IHC staining of Purified anti-mouse CD106 (Ep-CAM) (clone W21127K) on formalin-fixed paraffin-embedded C57BL/6 mouse testis tissue. Following antigen retrieval using 1X citrate buffer 1X (10 mM, pH 6.0) (Cat. No. 420901), the tissue was incubated without (panel A) or with (panel B) 1 µg/mL of purified anti-mouse CD106 (Ep-CAM) at 4°C overnight, followed by incubation with 2.5 µg/mL of Alexa Fluor® 647 goat anti-rat IgG (red) (Cat. No. 405416) for 1 hour at room temperature. The nuclei were counterstained with DAPI (blue) (Cat. No. 422801). The image was captured with a 10X objective. Scale bar: 50 µm
  • C. W21127K_PURE_CD106_IHCF_092523
    IHC staining of purified anti-mouse CD106 (Ep-CAM) (clone W21127K) on frozen mouse testis tissue. C57BL/6 mouse frozen testis section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then, the tissue was incubated 5 μg/mL of purified anti-mouse CD147 and without (panel A) or with (panel B) 5 µg/mL of purified anti-mouse CD106 (Ep-CAM) at 4°C overnight, followed by incubation 2.5 µg/mL of Alexa Fluor® 647 anti-rat IgG2a (red) (Cat. No. 407512) and Alexa Fluor® 594 anti-rat IgG1 (green) (Cat. No. 407410) for 1 hour at room temperature. The nuclei were counterstained with DAPI (blue) (Cat. No. 422801). The image was captured with a 10X objective. Scale bar: 50 µm
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117002 100 µg £76
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Description

CD106, also known as VCAM-1 and INCAM-110, is a 110 kD glycosylphosphatidylinositol (GPI)-linked transmembrane protein. It is constitutively expressed on bone marrow stromal cells, myeloid progenitors, splenic dendritic cells, activated endothelial cells, as well as some lymphocytes. CD106 expression can be upregulated on endothelial cells by inflammatory cytokines. CD106 is involved in adhesion and acts as a counter-receptor for VLA-4 (α4/β1 integrin) and LPAM-1 (α4/β7 integrin).  

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Transfectant cells (aa25-692) and recombinant protein (aa25-692)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-P, IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. For immunohistochemical staining on frozen tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application. 

Application Notes

Flow cytometry testing showed that clone W21127K does not affect the bindings of clones 429 (MVCAM.A) and W21127Q on target cells.

RRID
AB_3097228 (BioLegend Cat. No. 117002)

Antigen Details

Structure
Ig superfamily, 47 kD
Distribution

Bone marrow stromal cells, myeloid progenitors, splenic dendritic cells, activated endothelial cells

Function
Adhesion
Ligand/Receptor
VLA-4 (α4/β1 integrin) and LPAM-1 (α4/β7 integrin)
Cell Type
Dendritic cells, Endothelial cells, Mesenchymal Stem Cells
Biology Area
Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
  2. Kinashi T, et al. 1995. J Leukoc Biol. 57:168-73.
  3. Bevilacquea MP. 1993. Annu Rev Immunol. 11:767-804.
  4. Koni PA, et al. 2001. J Exp Med. 193:741-54.
Gene ID
22329 View all products for this Gene ID
UniProt
View information about CD106 on UniProt.org
Go To Top Version: 1    Revision Date: 09/07/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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