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Purified anti-mouse CD124 (IL-4Rα) Antibody

Pricing & Availability
Clone
I015F8 (See other available formats)
Regulatory Status
RUO
Other Names
IL-4R
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
I015F8_Purified_CD124_Antibody_FC_1_100312
C57BL/6 mouse splencotyes were stained with purified CD124 (clone I015F8) (top) or rat IgG2b, κ isotype control (bottom), followed by anti-rat IgG-PE and B220 APC.
  • I015F8_Purified_CD124_Antibody_FC_1_100312
    C57BL/6 mouse splencotyes were stained with purified CD124 (clone I015F8) (top) or rat IgG2b, κ isotype control (bottom), followed by anti-rat IgG-PE and B220 APC.
  • I015F8_Purified_CD124_Antibody_FC_2_100312
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144801 25 µg £70
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144802 100 µg £150
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Description

CD124, a subunit of IL-4R, is a 140 kD transmembrane glycoprotein. It associates with either the common γ-chain (CD132) to form the type I IL-4R complex, which specifically binds IL-4, or with IL-13Ra1 to form the type II IL-4R heterodimeric complex, which binds and transduces signals from either IL-4 or IL-13.  A truncated form of IL-4Rα exists in soluble form in biological fluids. CD124 is expressed on mouse late precursors and mature B cells, T cells, monocytes, and has low expression on granulocytes.  In B cells, CD124 can bind IL-4 and IL-13 and regulate IgE antibody production. In T cells, the type I IL-4R (IL-4R/gC) is mostly responsible for Th2 cell expansion by mediating IL-4-dependent activation of the transcription factors in hematopoietic cells. The type II IL-4R (IL-4R/IL-13Ra1) is the main route for nonhematopoietic cell responses to IL-4 or IL-13.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant Mouse IL4Rα encoding the extracellular domain (amino acid residues 26 - 233) was fused to the carboxy-terminal Fc region of human IgG1 via a linker peptide.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed
  2. Gubin MM, et al. 2018. Cell. 175:1014. PubMed
  3. Maier B, et al. 2020. Nature. 580:257. PubMed
RRID
AB_2561554 (BioLegend Cat. No. 144801)
AB_2561554 (BioLegend Cat. No. 144802)

Antigen Details

Structure
140 kD type I transmembrane glycoprotein, associates with common γ chain or IL-13 receptor alpha-1 subunit
Distribution

B cells, T cells, monocytes, and low levels on granulocytes.

Function
Regulates IgE antibody production in B cells, Th2 cell expansion, and IL-4 or IL-13 induced response in non-hematopoietic cells
Interaction
STAT6
Ligand/Receptor
IL-4, IL-13
Cell Type
B cells, Granulocytes, Monocytes, T cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Signal Transduction, Transcription Factors
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Kashiwada M, et al. 2001. J. Immunol. 167:6382.
2. Gilmour J, et al. 2008. J. Immunol. 124:437.
3. Hage T, et al. 1999. Cell 97:271.

Gene ID
16190 View all products for this Gene ID
UniProt
View information about CD124 on UniProt.org
Go To Top Version: 2    Revision Date: 11/22/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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