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Purified anti-mouse CD126 (IL-6Rα) Antibody

Pricing & Availability
Clone
W18166A (See other available formats)
Regulatory Status
RUO
Other Names
Interluekin 6 receptor alpha, CD126
Isotype
Rat IgG2a, κ
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Product Citations
publications
W18166A_PURE_CD169_Antibody_1_072320
C57BL/6 splenocytes were stained with CD3 FITC, CD4 PerCP/Cyanine5.5, and purified CD126 (IL-6Rα) (clone W18166A) (left) or rat IgG2a, κ isotype control (right), followed by anti-rat IgG PE. Dot plots are gated on CD3 positive cells
  • W18166A_PURE_CD169_Antibody_1_072320
    C57BL/6 splenocytes were stained with CD3 FITC, CD4 PerCP/Cyanine5.5, and purified CD126 (IL-6Rα) (clone W18166A) (left) or rat IgG2a, κ isotype control (right), followed by anti-rat IgG PE. Dot plots are gated on CD3 positive cells
  • W18166A_PURE_CD169_Antibody_2_072320
    Blocking of IL-6/STAT3 signaling pathway was assessed by intracellular staining of C57BL/6 splenocytes with STAT3 pY705 PE. Cells were incubated with purified CD126 (clone W18166A) or isotype control for 15 minutes, then treated with IL-6. Mean fluorescence intensity (MFI) of STAT3 pY705 PE were gated on CD4 positive cells.
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160402 500 µg £338
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Description

CD126 (IL-6Rα) is an 80kD member of the immunoglobulin superfamily that is expressed on activated T and B cells, monocytes, hepatocytes, and plasma cells. High affinity IL-6 receptors are formed by the non-covalent association of CD126 and the IL-6 receptor β chain (CD130 or gp130). CD126 binds IL-6 with low affinity, but does not signal. The β chain (gp130, CD130) does not bind IL-6 by itself, but associates with the α-chain/IL-6 complex to initiate signal transduction. IL-6 binding to the receptor complex results in the stimulation of B and T cells, and hematopoietic precursor proliferation and differentiation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse IL-6R recombinant protein, 1-357 a.a.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Block - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone W18166A binds to IL-6 receptor alpha, inhibits IL-6/IL-6 receptor interaction and mediated phosphorylation of signal transducer and activator of transcription 3 (STAT3). This clone does not cross-react with rat.

RRID
AB_2876570 (BioLegend Cat. No. 160402)

Antigen Details

Structure
Ig superfamily, associates with IL-6Rβ chain (CD130, gp130), 80 kD
Distribution

T cells and activated B cells, monocytes, hepatocytes, plasma cells

Function
Stimulates T cells, B cells, hematopoietic precursor proliferation and differentiation
Ligand/Receptor
IL-6
Cell Type
B cells, Epithelial cells, Fibroblasts, Granulocytes, Monocytes, Plasma cells, T cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Signal Transduction
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

Taga T, et al. 1997. Annu Rev Immunol. 15:797-819.

Fitzgerald K, et al. 2001. The Cytokine FactsBook. Academic Press London.

Boulanger MJ, et al. 2003. Science. 300:2101-4

Gene ID
16194 View all products for this Gene ID
UniProt
View information about CD126 on UniProt.org
Go To Top Version: 1    Revision Date: 07/17/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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