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Purified anti-mouse CD144 (VE-cadherin) Antibody

Pricing & Availability
Clone
VECD1 (See other available formats)
Regulatory Status
RUO
Other Names
Vascular endothelial-cadherin, VE-cadherin
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
VECD1_PE_042710
Mouse endothelial cell b.End.3 stained with purified VECD1 conjugated with PE
  • VECD1_PE_042710
    Mouse endothelial cell b.End.3 stained with purified VECD1 conjugated with PE
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138101 50 µg £61
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Description

CD144, also known as vascular endothelial-cadherin (VE-cadherin), is a 120 kD member of the type II Cadherin family.  It is an endothelial specific hemophilic adhesion molecule involved in endothelial cell survival, migration, contact-dependent growth inhibition and homophilic adhesion.  VE-cadherin is essential for maintaining the integrity of the endothelial barrier in vivo.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
VE-cadherin protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IP, WB, ICC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

It is also recommended using EDTA-based solutions for dissociating attachment-dependent cell lines.

Application Notes

Additional reported applications (for relevant formats) include: Immunoprecipitation1, Western Blot1, functional assay2, 3 by inhibiting vascular endothelial cell-cell interactions and immunocytochemistry staining of cultured cell lines4.

Application References

(PubMed link indicates BioLegend citation)
  1. Allport JR, et al. 2002. J. Leukoc. Biol. 71:821. (IP WB)
  2. Hirashima M, et al. 2009. Blood 93:1253. (Block)
  3. Matsuyoshi N, et al. 1997. Proc. Assoc. Am. Physicians 109:362. (Block)
  4. Matsumura K, et al. 2003. Blood 101:1367. (IF)
  5. Hirashima M, et al. 2009. Blood 101:2261. (FC)
  6. Urbschat A, et al. 2014. PLoS One. 9:84432. PubMed
Product Citations
  1. Brulois K, et al. 2020. Nat Commun. 3.095833333. PubMed
  2. Li GY, et al. 2023. J Pharm Anal. 13:262. PubMed
  3. Fischer D, et al. 2018. Front Pharmacol. 8:E10. PubMed
  4. Wang JC, et al. 2019. J Exp Clin Cancer Res. 38:235. PubMed
  5. Urbschat A, et al. 2014. PLoS One. 9:84432. PubMed
  6. Khaiboullina S, et al. 2019. Front Pharmacol. 10:642. PubMed
RRID
AB_10569116 (BioLegend Cat. No. 138101)

Antigen Details

Structure
A 120 kD member of type II Cadherin family
Distribution

Endothelial cells

Function
Involved in endothelial cell survival, migration, contact-dependent growth inhibition, and hemophilic adhesion.
Cell Type
Endothelial cells, Mesenchymal Stem Cells
Biology Area
Immunology, Innate Immunity, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Gotsch U, et al. 1997. J. Cell Sci. 110:583.
2. Kataoka H, et al. 1997. Dev. Growth Differ. 39:729.

Gene ID
12562 View all products for this Gene ID
UniProt
View information about CD144 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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