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Purified anti-mouse CD146 Antibody

Pricing & Availability
Clone
ME-9F1 (See other available formats)
Regulatory Status
RUO
Other Names
S-Endo 1 antigen, MUC18, MCAM, Mel-CAM, A32 antigen
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A_ME-9F1PE_080409.jpg
C57BL/6 splenocytes stained with ME-9F1 PE and NK-1.1 (PK136) APC
  • A_ME-9F1PE_080409.jpg
    C57BL/6 splenocytes stained with ME-9F1 PE and NK-1.1 (PK136) APC
  • B_ME-9F1_PE_080409.jpg
    C57BL/6 splenocytes stained with rat IgG2a (RTK2758) PE isotype control and NK-1.1 (PK136) APC
  • C_ME-9F1_PE_080409.jpg
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of CD146 (clone ME-9F1) purified, and CD3ε (clone 500A2) Alexa Fluor® 594 (green) overnight at 4°C, followed by 2.5 µg/ml of anti-rat IgG (clone Poly4054) Alexa Fluor® 647 (red) for 2 hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured by 10X objective.
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134701 50 µg £70
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134702 200 µg £213
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Description

CD146, also known as melanoma cell adhesion molecule (MCAM or Mel-CAM), MUC18, S-Endo1, and A32 antigen, is an integral membrane glycoprotein that belongs to the Ig superfamily. CD146 is strongly expressed by murine vascular endothelial cells. It is expressed on about 30% of neutrophils and 60% of NK cells. Unlike in humans, CD146 is undetectable on monocytes, dendritic cells, T cells, NKT cells, B cells, or smooth muscle cells in mouse. It has been reported that an increase in CD146 expression is associated with NK cell maturation. Combined with using CD27 and CD11b staining, CD146 may be an alternative marker to detect final stages of NK cell maturation and define NK cell subsets. CD146+ NK cells were found to be less cytotoxic and to produce less IFNγ than CD146- NK cells upon stimulation with target cells or activating antibodies. The role of CD146 on NK cell migration has yet to be investigated. The identification of CD146 ligand(s) will be crucial to address this issue.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Endothelial cell line TME-3H3
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Schrage A, et al. 2008. Histochem. Cell. Biol. 129:441.
  2. Birbrair A, et al. 2013. Am J Physiol Cell Physiol. 305:1098. PubMed
Product Citations
  1. Kemna J, et al. 2023. Nat Immunol. 24:414. PubMed
  2. Wang F, et al. 2023. Protein Cell. 14:105. PubMed
  3. Wang R, et al. 2023. Int J Biol Sci. 19:1490. PubMed
  4. Weng L, et al. 2016. Sci Rep. 6:38632. PubMed
  5. Hirai M, et al. 2016. Circ Res . 118: 20 - 28. PubMed
  6. Hu X, et al. 2016. Nat Commun. 7:13095. PubMed
  7. Grosse L, et al. 2020. Cell Metabolism. 32(1):87-99.e6. PubMed
  8. Dane EL, et al. 2022. Nat Mater. 21:710. PubMed
  9. Chen L, et al. 2020. Front Immunol. 11:584458. PubMed
  10. Breitbach M, et al. 2018. Cell Stem Cell. 1.098611111. PubMed
  11. Lim PJ, et al. 2019. Nat Metab. 0.402083333. PubMed
  12. Tsuchimoto A, et al. 2020. Sci Rep. 10:10796. PubMed
  13. Mierzejewski B, et al. 2020. Stem Cell Res Ther. 0.695138889. PubMed
  14. Guimarães‐Camboa N et al. 2017. Cell stem cell. 20(3):345-359 . PubMed
  15. Prideaux M, et al. 2021. Sci Rep. 11:22593. PubMed
  16. Birbrair A, et al. 2013. Am J Physiol Cell Physiol. 305:1098. PubMed
  17. Qin XY, et al. 2017. EBioMedicine. 24:257. PubMed
  18. Zhou H, et al. 2019. Genesis. :e23295. PubMed
  19. Luo Y, et al. 2017. Cell Res. 10.1038/cr.2017.8. PubMed
RRID
AB_1731991 (BioLegend Cat. No. 134701)
AB_1731991 (BioLegend Cat. No. 134702)

Antigen Details

Structure
CD146 is a transmembrane glycoprotein and belongs to the Ig superfamily of cell adhesion molecules.
Distribution

CD146 is strongly expressed by murine vascular endothelial cells, a subset of NK1.1+ cells, and neutrophils.

Function
CD146 has been reported to be functionally relevant for endothelial cell adhesion, angiogenesis, and NK cell function.
Ligand/Receptor
Unidentified ligand.
Cell Type
Endothelial cells, Mesenchymal Stem Cells, Neutrophils
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Despoix N, et al. 2008. Eur. J. Immunol. 38:2855.
2. Sorrentino A, et al. 2008. Exp. Hematol. 36:1035.
3. Bardin N, et al. 2009. Arterioscler. Thromb. Vasc. Biol. 29:746.

Gene ID
84004 View all products for this Gene ID
UniProt
View information about CD146 on UniProt.org
Go To Top Version: 2    Revision Date: 06/13/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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