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Purified anti-mouse CD169 (Siglec-1) Recombinant Antibody

Pricing & Availability
Clone
QA20A47 (See other available formats)
Regulatory Status
RUO
Other Names
Sialoadhesin, SER, Sialic acid-binding Ig-like lectin 1
Isotype
Rat IgG1, κ
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Product Citations
publications
QA20A47_PURE_CD169_Antibody_111422
C57BL/6 mouse bone marrow cells were stained with anti-mouse Ly-6G APC and purified anti-mouse CD169 (Siglec-1) recombinant (clone QA20A47) (left) or purified rat IgG1, κ isotype control (right) followed by anti-rat IgG1 PE.
  • QA20A47_PURE_CD169_Antibody_111422
    C57BL/6 mouse bone marrow cells were stained with anti-mouse Ly-6G APC and purified anti-mouse CD169 (Siglec-1) recombinant (clone QA20A47) (left) or purified rat IgG1, κ isotype control (right) followed by anti-rat IgG1 PE.
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138902 100 µg £161
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Description

CD169, also known as Siglec-1 and Sialoadhesin (Sn), is a type I lectin containing 17 immunoglobulin (Ig) domains (one variable domain and 16 constant domains). CD169 binds to sialic acids, which can be found on PSGL-1, CD43, CD206, and CD227. By its affinity to α2, 3-linked sialic acid, it is involved in macrophage binding to different cell types such as granulocytes, monocytes, NK, B, and T cells. CD169 was initially identified as a sialic acid-dependent sheep erythrocyte receptor (SER) on resident bone marrow cells of mice. It has been identified as highly expressed on resident bone marrow macrophages which plays an important role in retention of stem cells in mesenchymal stem cell niche. It is also found on some specific subsets of tissue macrophages in spleen, lymph nodes, bone marrow, liver, colon, lungs, and cancer cells. Evidence suggest that CD169-positive macrophages serve as lymph node-resident APCs to dominate early activation of tumor antigen-specific CD8+ T cells and invariant NK cell.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Recombinant
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2927983 (BioLegend Cat. No. 138902)

Antigen Details

Structure
Type I single membrane-spanning lectin containing 17 immunoglobulin (Ig) domains, belongs to the immunoglobulin superfamily
Distribution

Macrophages in spleen, lymph nodes, bone marrow, liver, colon and lungs

Function
Adhesion
Ligand/Receptor
PSGL-1, CD43, CD206 and CD227
Cell Type
Macrophages
Biology Area
Cell Biology, Immunology
Molecular Family
Adhesion Molecules, CD Molecules, Protein Kinases/Phosphatase, Siglec Molecules
Antigen References
  1. Chow A, et al. 2011. J Exp Med. 208:261-71.
  2. Asano K, et al. 2011. Immunity. 34:85-95.
  3. Xiong YS, et al. 2009. Clin Biochem. 42:1057-63.
  4. Varki A. 2009. Glycoconj J. 26:231-45.
  5. Rempel H, et al. 2008. PLoS One. 3:e1967.
  6. Crocker PR, et al. 2001. Trends Immunol. 22:337-42.
  7. Hartnell A, et al. 2001. Blood. 97:288-96.
  8. Crocker PR, et al. 1985. J Exp Med. 162:993-1014.
Gene ID
20612 View all products for this Gene ID
UniProt
View information about CD169 on UniProt.org
Go To Top Version: 1    Revision Date: 11/14/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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