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Purified anti-mouse CD183 (CXCR3) Antibody

Pricing & Availability
Clone
CXCR3-173 (See other available formats)
Regulatory Status
RUO
Other Names
CXCR3, G protein-coupled receptor 9 (GPR9), CKR-L2, IP10 receptor (IP10-R), Mig receptor (Mig-R )
Isotype
Armenian Hamster IgG
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Product Citations
publications
CXCR3-173_Pure_071408.jpg
C57BL/6 splenocytes stained with purified CXCR3-173, followed by anti-Armenian hamster IgG FITC and anti-mouse CD3 (17A2) APC
  • CXCR3-173_Pure_071408.jpg
    C57BL/6 splenocytes stained with purified CXCR3-173, followed by anti-Armenian hamster IgG FITC and anti-mouse CD3 (17A2) APC
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126502 200 µg £81
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Description

CD183, also known as CXCR3, is a member of the C-X-C chemokine family, characterized by a pair of cysteine residues separated by a single amino acid. CXCR3 is a 38 kD seven pass transmembrane receptor coupled to G-protein. It mediates Ca2+ mobilization and chemotaxis in response to C-X-C chemokines, such as IP10 (CXCL10), MIG (CXCL9), I-TAC (CXCL11) and PF4 (CXCL4). CXCR3 is expressed primarily on activiated T lymphocytes, NK cells, and some epithelial cells and endothelial cells. It is not expressed on B cells, monocytes, or granulocytes.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse N-terminus of CXCR3
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Block - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone CXCR3-173 does not work for Western blotting.7 For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 126526) with endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered.

Application References

(PubMed link indicates BioLegend citation)
  1. Krug A, et al. 2002. J. Immunol. 169:6079.
  2. McAleer JP, et al. 2009. J. Immunol. 182:5322. PubMed
  3. Sharma R. 2009. J. Immunol. 183:3212. (FC) PubMed
  4. Barbi J, et al. 2009. FASEB J. 23:3990. PubMed
  5. Kimpfler S, et al. 2009. J. Immunol. 183:6330. PubMed
  6. Dürr C, et al. 2010. Cancer Res. 70:10170. (Block) PubMed
  7. Uppaluri R, et al. 2008. Transplantation 86:137.
Product Citations
  1. Haworth O, et al. 2011. J Immunol. 186:6129. PubMed
  2. Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed
  3. Yu H, et al. 2020. Front Immunol. 11:1773. PubMed
  4. Hailemichael Y, et al. 2022. Cancer Cell. 40:509. PubMed
  5. Uzhachenko RV, et al. 2021. Cell Reports. 35(1):108944. PubMed
  6. Wei SC et al. 2017. Cell. 170(6):1120-1133 . PubMed
  7. Wei SC, et al. 2019. Immunity. 50:1084. PubMed
  8. Joseph R, et al. 2021. Br J Cancer. 125:176. PubMed
  9. Martin F, et al. 2011. Infect Immun. 79:1898. PubMed
  10. Dürr C, et al. 2010. Cancer Res. 70:10170. PubMed
  11. Pein M, et al. 2020. Nat Commun. 11:1494. PubMed
  12. Dionisio-Santos DA, et al. 2021. Front Neurosci. 15:758677. PubMed
  13. Zhu YP et al. 2018. Cell reports. 24(9):2329-2341 . PubMed
  14. Seung E, et al. 2011. J Immunol. 186:6830. PubMed
RRID
AB_1027635 (BioLegend Cat. No. 126502)

Antigen Details

Distribution

T cells, plasmacytoid dendritic cells, GM-CSF activated CD34+ hematopoietic progenitors

Function
Essential in T cell recruitment to sites of inflammation
Cell Type
Dendritic cells, Hematopoietic stem and progenitors, T cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Gene ID
12766 View all products for this Gene ID
UniProt
View information about CD183 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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