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Purified anti-mouse CD202b (Tie-2, CD202) Antibody

Pricing & Availability
Clone
W19424P (See other available formats)
Regulatory Status
RUO
Other Names
Tie-2, TEK
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W19424P_PURE_CD202b_Antibody_022422
bEnd.3 cells were stained with purified anti-mouse CD202b (Tie-2, CD202) (clone W19424P) (filled histogram) or rat IgG2b, κ isotype control (open histogram) followed by PE anti-rat IgG.
  • W19424P_PURE_CD202b_Antibody_022422
    bEnd.3 cells were stained with purified anti-mouse CD202b (Tie-2, CD202) (clone W19424P) (filled histogram) or rat IgG2b, κ isotype control (open histogram) followed by PE anti-rat IgG.
  • W19424P_PURE_CD202b_Antibody_2_022422
    C57 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 1 hour at room temperature. Then the section was stained with 10 µg/mL of purified anti-mouse CD202b (Tie-2, CD202) (clone W19424P) at 4°C overnight. On the next day, tissue was incubated with Alexa Fluor® 594 goat anti-rat IgG (clone Poly4054) (red) and anti-mouse CD3ε (clone 500A2) Alexa Fluor® 647 (green) for an hour at room temperature. Nuclei were counter-stained with DAPI (blue). The image was captured with a 10X object.
  • W19424P_PURE_CD202b_Antibody_3_022422
    C57 mouse frozen kidney section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 1 hour at room temperature. Then the section was stained with 10 µg/mL of purified anti-mouse CD202b (Tie-2, CD202) (clone W19424P) at 4°C overnight. On the next day, tissue was incubated with Alexa Fluor® 594 goat anti-rat IgG (clone Poly4054) (red) for an hour at room temperature. Nuclei were counter-stained with DAPI (blue). The image was captured with a 10X object.
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110402 100 µg £89
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Description

CD202b, also known as Tie-2 or TEK, is a 145 kD type I transmembrane protein. It is a member of the receptor tyrosine kinase (RTK) family of proteins and is expressed by endothelial cells and their progenitors, quiescent hematopoietic stem cells (HSCs), and a subpopulation of monocytes. Angiopoietin-1 (Ang-1) is an activator of CD202b to promote, maintain, and stabilize mature vessels and to maintain HSCs in the quiescent state. Ang-2 is another ligand of CD202b, which is involved in postnatal angiogenesis and in antagonizing the effects of Ang-1. Tie-2 also binds to Ang-4.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For immunohistochemistry on frozen tissue sections, a concentration range of 5 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2922458 (BioLegend Cat. No. 110402)

Antigen Details

Structure
The receptor tyrosine kinase (RTK) family
Distribution

Endothelial cells and their progenitors, quiescent hematopoietic stem cells (HSCs), and a subpopulation of monocytes

Cell Type
Endothelial cells, Epithelial cells, T cells
Biology Area
Angiogenesis, Cell Biology, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Fathers KE, et al. 2005. Am. J. Pathol. 167:1753-62.
  2. Yano M, et al. 1997. Blood 89:4317-26.
  3. Kolatsi-Joannou M, et al. 2001. Dev. Dyn. 222:120-6.
Gene ID
21687 View all products for this Gene ID
UniProt
View information about CD202b on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 02/24/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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