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Purified anti-mouse CD204 Antibody

Pricing & Availability
Clone
7G5C33 (See other available formats)
Regulatory Status
RUO
Other Names
Macrophage scavenger receptor 1 (Msr1), Scavenger receptor type A (SR-A), SRA
Isotype
Rat IgG1, κ
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Product Citations
publications
7G5C33_Purified_CD204_Antibody_WB_012419
Total cell lysates (15 µg total protein) from NIH/3T3 (negative control), Raw264.7, and THP-1 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL of Purified anti-CD204 Antibody, clone 7G5C33, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
  • 7G5C33_Purified_CD204_Antibody_WB_012419
    Total cell lysates (15 µg total protein) from NIH/3T3 (negative control), Raw264.7, and THP-1 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL of Purified anti-CD204 Antibody, clone 7G5C33, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
  • 7G5C33_Purified_CD204_Antibody_WB_042415
    Total cell lysate from THP-1 cells (lane 1) and RAW264.7 cells (lane 2) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-mouse CD204 (clone 7G5C33) antibody. Proteins were visualized using an anti-rat-IgG secondary conjugated to HRP and chemiluminescence detection.
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676202 100 µg £182
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Description

CD204, also known as scavenger receptor A (SR-A) and the macrophage scavenger receptor (MSR), is one of the phagocytic pattern-recognition receptors (PRRs) expressed on macrophages and dendritic cells. CD204 was initially identified as a receptor mediating recognition and internalization of low-density lipoprotein (LDL) by macrophages and playing critical roles in atherogenesis. Recent studies have revealed that CD204 recognizes apoptotic cells, modified lipid proteins, and exogenous pathogen-associated molecular patterns (PAMPs), which results in the induction of innate immune and inflammatory responses. CD204 can act as a co-receptor for Toll-like receptors, such as TLR3, TLR4, or TLR9, to facilitate the expression of proinflammatory cytokines. CD204 has been implicated in several pathological processes such as Alzheimer’s disease, sepsis, ischemic injury, and coronary artery disease.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial mouse CD204 recombinant protein (79-458 a.a.) expressed in 293E cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

CD204 is polymorphic and clone 7G5C33 may not react with all mouse strains. In house testing with this clone showed no reactivity to C57BL/6 mouse spleen samples.

The predicted molecular weight of CD204 is 50 kD, the observed molecular weight when clone 7G5C33 is used for western blot is ~70 kD. This is likely due to glycosylation of CD204 at multiple sites.

RRID
AB_2565623 (BioLegend Cat. No. 676202)

Antigen Details

Structure
485 amino acids with a predicted molecular weight of 50 kD and is a single-pass type II membrane protein.
Distribution

Plasma membrane, low-density lipoprotein particle, and endocytic vesicle emmbrane.

Function
CD204, a trimeric type II integral membrane glycoprotein, is predominantly expressed on macrophages and dendritic cells. CD204 plays important roles in lipid metabolism, host defense, and inflammatory responses.
Interaction
CD204 interacts with HSPA1A.
Biology Area
Cell Biology, Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Kelley JL, et al. 2014. Crit. Rev. Immunol. 34:241.
2. Saito Y, et al. 2014. J. Clin. Exp. Hematop. 54:59.
3. Jozefowski S, et al. 2014. Innate Immun. 20:826.
4. Haasken S, et al. 2013. J. Immunol. 191:1055.
5. Yu H, et al. 2012. Biochim. Biophys. Acta. 7:1192.
6. Yu X, et al. 2012. Oncoimmunology 5:770.

Gene ID
20288 View all products for this Gene ID
UniProt
View information about CD204 on UniProt.org
Go To Top Version: 1    Revision Date: 04/24/2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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