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Purified anti-mouse CD321 (F11R) Antibody

Pricing & Availability
Clone
27-9 (See other available formats)
Regulatory Status
RUO
Other Names
Junctional adhesion molecule-1,Jam-1, Jam-A, junctional adhesion molecule-A, CD321
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
27-9_PURE_CD321_Antibody_1_112221
C57BL/6 platelets were stained with anti-mouse CD41 APC and purified anti-mouse CD321 (F11R) (clone 27-9) purified (left) or rat IgG2a, κ isotype control (right), followed by anti-rat IgG PE.
  • 27-9_PURE_CD321_Antibody_1_112221
    C57BL/6 platelets were stained with anti-mouse CD41 APC and purified anti-mouse CD321 (F11R) (clone 27-9) purified (left) or rat IgG2a, κ isotype control (right), followed by anti-rat IgG PE.
  • 27-9_PURE_CD321_Antibody_2_112221
    C57BL/6 mouse frozen kidney section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. The section was stained with 5 µg/mL with purified anti-mouse CD321 (F11R) (clone 27-9) followed by anti-mouse IgG Alexa Fluor® 555. Nuclei were counterstained with DAPI (blue). The image was captured by 40X objective.
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107802 100 µg £206
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Description

F11R is a cell adhesion molecule and immunoglobulin superfamily member and an important regulator of tight junction assembly in epithelia. It is found on the surface of platelets, and plays a role in platelet aggregation, secretion, adhesion, and spreading. By interacting with integrin alpha-L/beta-2, it is involved in leukocyte transmigration. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
E14.5 mouse fetal liver cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.5 µg per million cells in 100 µL volume. For immunohistochemistry, a concentration range of 2.0 - 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Sugano Y et al. 2008. Blood. 111(3): 1167–72.
RRID
AB_2904280 (BioLegend Cat. No. 107802)

Antigen Details

Structure
32 kD single pass type I membrane glycoprotein, member of the immunoglobin superfamily
Distribution

Cell membrane, tight junctions

Function
Protein homodimerization, integrin binding, PDZ domain binding, tight junction formation
Interaction
integrin alpha-L/beta-2, MPDZ, PARD3, ITGAL
Ligand/Receptor
integrin alpha-L/beta-2
Cell Type
Endothelial cells, Epithelial cells, Leukocytes, Platelets
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Martin-Padura I, et al. 1998. J. Cell Biol. 142:117-27.
  2. Muller W.A. 2003. Trends Immunol. 24:327-34.
  3. Kostrewa D., et al. 2001. EMBO J. 20:4391-8.
Gene ID
O88792 View all products for this Gene ID
UniProt
View information about CD321 on UniProt.org
Go To Top Version: 1    Revision Date: 11/22/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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