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Purified anti-mouse CD339 (Jagged 1) Antibody

Pricing & Availability
Clone
HMJ1-29 (See other available formats)
Regulatory Status
RUO
Other Names
JAG1, Jagged1
Isotype
Armenian Hamster IgG
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Product Citations
publications
HMJ1-29_Purified_HMJ1-29_Antibody_FC_072313
Mouse Jagged 1 transfected CHO cells were stained with purified HMJ1-29 (filed histogram) or purified Armenian hamster IgG isotype control (open histogram), followed by anti-hamster IgG PE.
  • HMJ1-29_Purified_HMJ1-29_Antibody_FC_072313
    Mouse Jagged 1 transfected CHO cells were stained with purified HMJ1-29 (filed histogram) or purified Armenian hamster IgG isotype control (open histogram), followed by anti-hamster IgG PE.
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130902 500 µg £221
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Description

CD339, also known as Jagged 1, is a 135 kD type 1 transmembrane protein, with one DSL (Delta, Serrate, LagZ) domain, fifteen EGF-like domains, and one cysteine-rich region. Jagged 1 is expressed by many cell types including macrophages, subsets of dendritic cells, astrocytes, stromal, and epithelial cells. CD339 regulates cell fate decisions in various tissues, and has an important role in neurogenesis and development of the cardiovascular system. Studies suggest Jagged 1 is involved in Th2 polarization and Treg induction.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Murine Jagged 1-Fc fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: in vivo blocking of Jagged 1 in EAE1 and immunohistochemical staining of frozen spleen sections2.

Application References

(PubMed link indicates BioLegend citation)
  1. Elyaman W, et al. 2007. J. Immunol. 179:5990. (Block)
  2. Sekine C, et al. 2009. Int. Immunol. 21:295. (IHC)
Product Citations
  1. Duran CL, et al. 2023. Breast Cancer Res. 25:37. PubMed
  2. Enriquez AB, et al. 2022. iScience. 25:104305. PubMed
  3. Martinez-Lozada Z, et al. 2020. Neurochemistry International. 139:104787. PubMed
  4. Enriquez AB, et al. 2022. iScience. 25:104305. PubMed
  5. Sharma VP, et al. 2021. Nat Commun. 12:7300. PubMed
RRID
AB_2561301 (BioLegend Cat. No. 130902)

Antigen Details

Structure
Type 1 transmembrane protein, 1 DSL (Delta, Serrate, LagZ) domain, 15 EGF-like domains, 1 cystein riche region; 135 kD
Distribution

Macrophages, subsets of dendritic cells, stromal cells, epithelial cells, astrocytes

Function
Regulates cell fate decisions in various tissues, important role in cardiovascular system development, neurogenesis, and T cell polarization to Th2 lineage
Ligand/Receptor
Notch1, Notch2, Notch3
Cell Type
Astrocytes, Dendritic cells, Epithelial cells, Macrophages
Biology Area
Angiogenesis, Cell Biology, Immunology
Molecular Family
CD Molecules
Antigen References

1. Amsen D, et al. 2004. Cell. 117:515.
2. Cheng P, et al. 2007. Blood. 109: 507. 
3. Goh F, et al. 2009. Immunology. 127:326. 
4. Zeng H, et al. 2012. PLoS One. 7:e35905.

Gene ID
16449 View all products for this Gene ID
UniProt
View information about CD339 on UniProt.org
Go To Top Version: 2    Revision Date: 08/28/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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