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Purified anti-mouse CD365 (Tim-1) Antibody

Pricing & Availability
Clone
3B3 (See other available formats)
Regulatory Status
RUO
Other Names
T cell immunoglobulin and mucin domain containing protein-1, T cell and airway phenotype regulator (Tapr), hepatitic virus cellular receptor 1, CD365
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
3B3_PURE_CD365_Antibody_040616
Mouse Tim-1 transfected cells were stained with purified anti-mouse CD365 (clone 3B3, filled histogram) or purified rat IgG2a, κ isotype control (open histogram), followed by anti-rat IgG PE.
  • 3B3_PURE_CD365_Antibody_040616
    Mouse Tim-1 transfected cells were stained with purified anti-mouse CD365 (clone 3B3, filled histogram) or purified rat IgG2a, κ isotype control (open histogram), followed by anti-rat IgG PE.
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151702 100 µg £145
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Description

CD365 (Tim-1) is a transmembrane protein also known as T cell immunoglobulin and mucin domain containing protein-1 and hepatitis virus cellular receptor 1. It is developmentally expressed at high levels in the blastocyst. Tim-1 is expressed on activated CD4+ lymphocytes especially on Th2 cells and has been implicated to play a critical role in the development of atopic disease and other Th2-biased immune responses. Tim-1 is hepatitis A virus receptor in humans. Tim-4 is the endogenous ligand of Tim-1. The interaction of Tim-1 and Tim-4 is involved in costimulation of T cell proliferation. Tim-1 is an endogenous ligand for LMIR5/CD300b.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse TIM-1 (igV domain) FC chimera IFA
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Direct ELISA - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.75 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Fonseca JA, et al. 2016. PLoS One. e0154819:11. PubMed
  2. Wagner AK, et al. 2017. Nat Commun. 8:15627. PubMed
RRID
AB_2616785 (BioLegend Cat. No. 151702)

Antigen Details

Structure
Transmembrane protein containing immunoglobulin domain and mucin-like domain with the predicted molecular weight of 33 kD.
Distribution

Developmentally regulated; highly expressed in embryonic development in blastocysts. Expressed on activated CD4+ lymphocytes, especially Th2 cell lines.

Function
Binds hepatitis virus, may play a critical role in immune responses and the development of atopic diseases (in particular airway hyperreactivity).
Ligand/Receptor
Binds hepatitis A virus in humans, Tim-4, and LMIR5/CD300b.
Biology Area
Cell Biology, Cell Proliferation and Viability, Immunology
Molecular Family
CD Molecules, TCRs
Antigen References

1. McIntire JJ, et al. 2001. Nat. Immunol. 2:1109.
2. Kuchroo VK, et al. 2003. Nat. Rev. Immunol. 3:454.
3. Wills-Karp M, et al. 2001. Nat. Rev. Immunol. 1:69.
4. Meyers JH, et al. 2005. Nat. Immunol. 6(5):455.
5. Umetsu S, et al. 2005. Nat. Immunol. 6:447.
6. Lee HH, et al. 2010. J. Immunol. 185:5225.

Gene ID
171283 View all products for this Gene ID
UniProt
View information about CD365 on UniProt.org
Go To Top Version: 1    Revision Date: 04/15/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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