Purified anti-mouse CD45.2 Maxpar Ready Antibody anti-CD45.2

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Clone: 104 (See other available formats)
Regulatory Status: RUO
Other Names: Ly-5.2, LCA
Isotype: Mouse (SJL) IgG2a, κ
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Product Citations: publications

104_Purified_CD45.2_Cytof_060414.jpg — C57BL/6 mouse bone marrow cells stained with 147Sm-anti-CD45.2 (104). Data provided by DVS Sciences.

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109843

100 µg

£73

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Description

CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: B10.S mouse thymocytes and splenocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1.0 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
CyTOF® - Verified
Recommended Usage: This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.
Application Notes: The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation⁴, in vivo and in vitro blocking of B cell responses^1,2, and immunohistochemical staining of acetone-fixed frozen sections³.
Additional Product Notes: Maxpar® is a registered trademark of Standard BioTools Inc.
RRID: AB_2563751 (BioLegend Cat. No. 109843)

Antigen Details

Structure: Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution: All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice
Function: Phosphatase, T and B cell activation
Ligand/Receptor: Galectin-1, CD2, CD3, CD4
Biology Area: Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules
Antigen References: 1. Suzuki K, et al. 2000. Immunity 13:691.
Gene ID: 19264 View all products for this Gene ID
UniProt: View information about CD45.2 on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Related FAQs

Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?

We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm^®. Please contact Fluidigm^® directly for additional data and further details.

http://techsupport.fluidigm.com/

Can I use Maxpar® Ready format clones for flow cytometry staining?

We have not tested the Maxpar^® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.

I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.

We only supply the antibody and not test that in house. Please contact Fluidigm^® directly for troubleshooting advice: http://techsupport.fluidigm.com/

Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?

The Maxpar^® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.

Other Formats

View All CD45.2 Reagents Request Custom Conjugation

Description	Clone	Applications
Biotin anti-mouse CD45.2	104	FC
FITC anti-mouse CD45.2	104	FC
PE anti-mouse CD45.2	104	FC
Purified anti-mouse CD45.2	104	FC,Block,IHC-F,IP
APC anti-mouse CD45.2	104	FC
Alexa Fluor® 488 anti-mouse CD45.2	104	FC
Alexa Fluor® 647 anti-mouse CD45.2	104	FC
Pacific Blue™ anti-mouse CD45.2	104	FC
Alexa Fluor® 700 anti-mouse CD45.2	104	FC
APC/Cyanine7 anti-mouse CD45.2	104	FC
PerCP anti-mouse CD45.2	104	FC
PerCP/Cyanine5.5 anti-mouse CD45.2	104	FC
PE/Cyanine7 anti-mouse CD45.2	104	FC
Brilliant Violet 421™ anti-mouse CD45.2	104	FC
Brilliant Violet 570™ anti-mouse CD45.2	104	FC
Brilliant Violet 650™ anti-mouse CD45.2	104	FC
Brilliant Violet 510™ anti-mouse CD45.2	104	FC
Brilliant Violet 785™ anti-mouse CD45.2	104	FC
Brilliant Violet 605™ anti-mouse CD45.2	104	FC
Purified anti-mouse CD45.2 (Maxpar® Ready)	104	FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD45.2	104	FC
Brilliant Violet 711™ anti-mouse CD45.2	104	FC
Alexa Fluor® 594 anti-mouse CD45.2	104	IHC-F,SB
APC/Fire™ 750 anti-mouse CD45.2	104	FC
TotalSeq™-A0157 anti-mouse CD45.2	104	PG
TotalSeq™-B0157 anti-mouse CD45.2	104	PG
TotalSeq™-C0157 anti-mouse CD45.2	104	PG
Brilliant Violet 750™ anti-mouse CD45.2	104	FC
Spark Blue™ 550 anti-mouse CD45.2	104	FC
Spark NIR™ 685 anti-mouse CD45.2	104	FC
Spark UV™ 387 anti-mouse CD45.2	104	FC
Spark Blue™ 574 anti-mouse CD45.2 (Flexi-Fluor™)	104	FC
Spark Red™ 718 anti-mouse CD45.2 (Flexi-Fluor™)	104	FC

Certificate of Analysis

Go To Top Version: 2 Revision Date: 07/21/2022

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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