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Purified anti-mouse CD63 Antibody

Pricing & Availability
Clone
NVG-2 (See other available formats)
Regulatory Status
RUO
Other Names
LIMP, LAMP-3, gp55, melanoma-associated antigen, ME491
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
a-NVG-2_Pure_CD63_Antibody_1_040620
Thioglycolate-elicited BALB/c mouse peritoneal macrophages were fixed, permeabilized and intracellularly stained with purified CD63 (clone NVG-2) (left) or purified rat IgG2a, κ isotype control (right), followed by biotinylated anti Rat IgG, SAV PE, and CD11b APC.
  • a-NVG-2_Pure_CD63_Antibody_1_040620
    Thioglycolate-elicited BALB/c mouse peritoneal macrophages were fixed, permeabilized and intracellularly stained with purified CD63 (clone NVG-2) (left) or purified rat IgG2a, κ isotype control (right), followed by biotinylated anti Rat IgG, SAV PE, and CD11b APC.
  • b-NVG-2_Pure_041312
    bEnd.3, mouse endothelial cells were stained with purified CD63 (clone NVG-2) (filled histogram) or rat IgG2a, κ isotype control (open histogram), followed by anti-rat IgG PE.
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143901 25 µg £61
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143902 100 µg £141
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Description

CD63, also known as LIMP, LAMP-3, gp55, and melanoma-associated antigen (ME491), is a member of the tetraspanin superfamily (TM4SF) that constitutes a main component of the lysosomal membrane. It is expressed on activated platelets, monocyte/macrophages, endothelium, fibroblasts, osteoblasts, and smooth muscle cells. CD63 may be involved in platelet activation and is thought to function as a transmembrane adaptor protein. CD63 has been shown to associate with CD9, CD81, VLA-3, and VLA-6. In mice, there are two CD63 gene loci, of which only one is functional. CD63 deficient mice are viable, and there is no alteration in the population of immune cells.  A recent report shows that CD63-deficient mice exhibit a significant reduction in both leukocyte rolling and recruitment in a peritonitis model. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Intestinal lamina propria light-density cells (enriched with eosinophils)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICFC - Quality tested
FC - Verified
WB, IHC-F - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For intracellular flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: Western blotting1 and immunofluorescence1.

Application References

(PubMed link indicates BioLegend citation)
  1. Verjan Garcia N, et al. 2011. J. Immunol. 187:2268. (WB, IF)
Product Citations
  1. Ma Y, et al. 2021. Sci Rep. 11:13471. PubMed
  2. Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed
  3. Qi L, et al. 2023. J Biol Chem. 299:102775. PubMed
  4. Hirata T, et al. 2023. iScience. 26:105747. PubMed
  5. Gomes P, et al. 2023. Cell Commun Signal. 21:35. PubMed
  6. Khan NZ, et al. 2021. Brain Behav Immun. 92:165. PubMed
  7. Zhang W, et al. 2022. Dev Cell. 57:329. PubMed
  8. Daemen S, et al. 2021. Cell Reports. 34(2):108626. PubMed
  9. Arnold IC, et al. 2018. J Exp Med. 215:2055. PubMed
  10. Crawford G, et al. 2018. Nat Immunol. 1.388194444. PubMed
  11. Wang G, et al. 2021. Nat Commun. 12:5733. PubMed
  12. Cappariello A, et al. 2018. J Bone Miner Res. 33:517. PubMed
  13. Elgawidi A, et al. 2020. Med Microbiol Immunol. 209:473. PubMed
  14. O'Dea KP, et al. 2020. J Extracell Vesicles. 9:1706708. PubMed
  15. Jiang L, et al. 2020. Microorganisms. 9:. PubMed
  16. Nakai W, et al. 2016. Sci Rep. 6: 33935. PubMed
  17. Samoylenko A, et al. 2021. Sci Rep. 11:19594. PubMed
  18. Teng Y, et al. 2017. Nat Commun. 8:14448. PubMed
  19. Daemen S, et al. 2021. STAR Protoc. 2:100511. PubMed
  20. Gubin MM, et al. 2018. Cell. 175:1014. PubMed
  21. Lobastova L, et al. 2021. Frontiers in Cell and Developmental Biology. 9:698503. PubMed
  22. Sudhakar JN, et al. 2020. Nat Commun. 3.434722222. PubMed
  23. Lloyd-Lewis B, et al. 2018. J Biol Chem. 9:2684. PubMed
  24. Degosserie J, et al. 2018. J Extracell Vesicles. 11:243. PubMed
  25. Garcia-Martin R, et al. 2022. Nature. 601:446. PubMed
  26. Teng Y, et al. 2021. Mol Ther. 29:2424. PubMed
  27. Harada Y, et al. 2020. Cell Rep. 33:108261. PubMed
  28. Garcia-Martin R, et al. 2022. Cell Rep. 38:110277. PubMed
  29. Cappariello A, et al. 2022. Calcif Tissue Int. Online ahead of print. PubMed
  30. Ruan Z, et al. 2022. Cell Rep. 39:110791. PubMed
RRID
AB_11204263 (BioLegend Cat. No. 143901)
AB_11204263 (BioLegend Cat. No. 143902)

Antigen Details

Structure
Tetraspan transmembrane superfamily (TM4SF), type III lysosomal glycoprotein, 53 kD
Distribution

Activated platelets, monocytes, macrophages, endothelium, fibroblasts, osteoclasts, and smooth muscle cells

Function
Platelet activation
Interaction
CD9, CD81, VLA-3, and VLA-6
Cell Type
Endothelial cells, Fibroblasts, Macrophages, Monocytes, Osteoclasts, Platelets
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Azorsa DO, et al. 1991. Blood 78:280.
2. Kishimoto T, et al. 1997. Leukocyte Typing V1. Oxford University Press New York.
3. Hildreth JE, et al. 1991. Blood 77:121.

Gene ID
12512 View all products for this Gene ID
UniProt
View information about CD63 on UniProt.org
Go To Top Version: 3    Revision Date: 04/06/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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