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Purified anti-mouse CD80 Antibody

Pricing & Availability
Clone
16-10A1 (See other available formats)
Regulatory Status
RUO
Other Names
B7-1, B7, Ly-53
Isotype
Armenian Hamster IgG
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Product Citations
publications
16-10A1_Purified_CD80_Antibody_FC_090414.jpg
LPS-stimulated (3 days) BALB/c splenocytes stained with purified CD80 (clone 16-10A1, filled histogram) or Armenian hamster IgG isotype control (open histogram), followed by goat anti-Armenian hamster IgG PE.
  • 16-10A1_Purified_CD80_Antibody_FC_090414.jpg
    LPS-stimulated (3 days) BALB/c splenocytes stained with purified CD80 (clone 16-10A1, filled histogram) or Armenian hamster IgG isotype control (open histogram), followed by goat anti-Armenian hamster IgG PE.
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104702 500 µg £95
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Description

CD80 is a 60 kD highly glycosylated protein. It is a member of the Ig superfamily and is also known as B7-1, B7, and Ly-53. CD80 is constitutively expressed on dendritic cells and monocytes/macrophages, and inducibly expressed on activated B and T cells. The ligation of CD28 on T cells with CD80 and CD86 (B7-2) on antigen presenting cells (such as dendritic cells, macrophages, and B cells) elicits co-stimulation of T cells resulting in enhanced cell activation, proliferation, and cytokine production. CD80 appears to be expressed later in the immune response than CD86. CD80 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Reported Reactivity
Dog
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
CHO cell line transfected with mouse B7 (CD80)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Block, IHC-F, IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation2, in vitro and in vivo blocking of CTLA-4 Ig to CD80 by blocking costimulation of T cells by activated B cells2-4, and immunohistochemical staining of acetone-fixed frozen sections1,4. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 104747-104752).

Application References

(PubMed link indicates BioLegend citation)
  1. Harlan DM, et al. 1994. P. Natl. Acad. Sci. USA 91:3137. (IHC)
  2. Razi-Wolf Z, et al. 1992. P. Natl. Acad. Sci. USA 89:4210. (Block, IP)
  3. Hathcock KS, et al. 1994. J. Exp. Med. 180:631. (Block)
  4. Herold KC, et al. 1997. J. Immunol. 158:984. (Block, IHC)
  5. Ma XT, et al. 2006. Cancer Res. 66:1169.
  6. Andoniou CE, et al. 2005. Nature Immunology 6:1011. (FC)
  7. Lawson BR, et al. 2007. J. Immunol. 178:5366.
  8. Turnquist HR, et al. 2007. J. Immunol. 178:7018.
  9. Misra RS, et al. 2010. J. Exp Med. 207:1775. PubMed
  10. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  11. Philipsen L, et al. 2013. Mol Cell Proteomics. 12:2551. PubMed
Product Citations
  1. Jayachandran R, et al. 2019. Immunity. 50:152. PubMed
  2. Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed
  3. Paterson MJ, et al. 2020. PLoS Pathog. 16:e1008733. PubMed
  4. Cai S, et al. 2020. Sci Rep. 10:14249. PubMed
  5. Malinczak CA, et al. 2021. J Immunol. 206:1315. PubMed
  6. Moore AR, et al. 2022. Cell Rep. 41:111651. PubMed
  7. Wan Mohd Zawawi WFA, et al. 2021. Sci Rep. 11:10278. PubMed
  8. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  9. Kouwenberg M, et al. 2020. PLoS One. 15:e0230835. PubMed
  10. Niss Arfelt K, et al. 2017. Blood. 129:866. PubMed
  11. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  12. Chen L, et al. 2020. Front Immunol. 11:584458. PubMed
  13. Forsyth KS, et al. 2020. PLoS Pathog. 16:e1008685. PubMed
  14. Zhao L, et al. 2018. Nat Med. 24:1536. PubMed
  15. Ruer-Laventie J, et al. 2020. Bio Protoc. e3531:10. PubMed
  16. Hu G, et al. 2021. Nat Commun. 12:773. PubMed
  17. Chen HW, et al. 2021. Biomedicines. 9:. PubMed
  18. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  19. Hammer A, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01922. PubMed
  20. Song K, et al. 2017. Oncotarget. 8:38554. PubMed
  21. Akiel M, et al. 2017. Cancer Res. 77:4014. PubMed
  22. Roussey JA, et al. 2017. J Immunol. 199:3535. PubMed
  23. Wu J, et al. 2017. Am J Pathol. 10.1016/j.ajpath.2016.12.021. PubMed
  24. Scortegagna M, et al. 2020. Nat Commun. 11:99. PubMed
  25. Lou F, et al. 2020. Immunity. 53(1):204-216.e10. PubMed
  26. Andrews AR, et al. 2019. Cancer Cell Int. 0.955555556. PubMed
  27. Diao J, et al. 2018. J Immunol. 201:1306. PubMed
  28. Khameneh HJ, et al. 2017. J Immunol. 198:196. PubMed
  29. Prabakaran T, et al. 2021. EBioMedicine. 66:103314. PubMed
  30. Klinger M, et al. 2007. Am J Physiol Regul Integr Comp Physiol. 293:R677. PubMed
RRID
AB_313123 (BioLegend Cat. No. 104702)

Antigen Details

Structure
Ig superfamily, 60 kD
Distribution

Macrophages, activated B cells and T cells, dendritic cells

Function
T cell costimulation
Ligand/Receptor
CD28 (stimulatory), CD152(CTLA4) (inhibitory)
Cell Type
B cells, Dendritic cells, Macrophages, T cells, Tregs
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Linsley PS, et al. 1991. J. Exp. Med. 174:561.
3. Salomon B, et al. 2001. Annu. Rev. Immunol. 19:225.

Gene ID
12519 View all products for this Gene ID
UniProt
View information about CD80 on UniProt.org

Related FAQs

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Go To Top Version: 4    Revision Date: 08/11/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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