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Purified anti-mouse CD90.2 (Thy1.2) (Maxpar® Ready) Antibody

Pricing & Availability
Clone
30-H12 (See other available formats)
Regulatory Status
RUO
Other Names
Thy-1.2
Isotype
Rat IgG2b, κ
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Product Citations
publications
30-H12_Purified_CD90.2_Cytof_061014.jpg
C57BL/6 mouse bone marrow cells stained with 156Gd-anti-CD90.2 (Thy-1.2) (30-H12). Lymphocytes (top) and myeloid cells (bottom) are displayed in the analysis. Data provided by DVS Sciences.
  • 30-H12_Purified_CD90.2_Cytof_061014.jpg
    C57BL/6 mouse bone marrow cells stained with 156Gd-anti-CD90.2 (Thy-1.2) (30-H12). Lymphocytes (top) and myeloid cells (bottom) are displayed in the analysis. Data provided by DVS Sciences.
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105333 100 µg £126
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Description

CD90.2 is a 25-35 kD immunoglobulin superfamily member also known as Thy1.2. It is expressed on hematopoietic stem cells and neurons, all thymocytes, and peripheral T cells in Thy1.2 bearing mouse strains (Balb/c, CBA/J, C3H/He, C57BL/-, DBA, NZB/-). CD90.2 is a glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein involved in signal transduction. CD90.2 is involved in costimulation of lymphocyte proliferation and induction of hematopoietic stem cells differentiation. CD90.2 has been shown to interact with CD45. The 30-H12 antibody has been reported to induce Ca2+ flux in thymocytes and, in combination with antibody against the CD3/TCR complex, promote thymocyte apoptosis and inhibit CD3-mediated proliferative responses of mature T lymphocytes.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
CyTOF® - Verified
Recommended Usage

This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.

Application Notes

Additional reported applications (for the relevant formats) include: in vivo and in vitro depletion1,2,7, costimulation of CD3/TCR-mediated signal transduction3,4, and immunohistochemical staining5 of acetone-fixed frozen sections. The 30-H12 antibody does not react with Thy-1.1 alloantigen of the AKR/J and PL strains. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301 & 101302) is recommended prior to immunofluorescent staining. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 105351 & 105352).

Additional Product Notes

Maxpar® is a registered trademark of Standard BioTools Inc.

Application References

(PubMed link indicates BioLegend citation)
  1. Hathcock KS. 1991. Current Protocols in Immunology. 3.4.1. (Deplete)
  2. Seaman WE. 1983. J. Immunol. 130:1713. (Deplete)
  3. Nakashima I, et al. 1991. J. Immunol. 147:1153. (Costim)
  4. Nakashima I, et al. 1993. J. Immunol. 151:3511. (Costim)
  5. Ledbetter JA, et al. 1980. J. Exp. Med. 152:280. (IHC)
  6. Hardy B, et al. 2005. Int. Immunol. 17:615.
  7. Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
  8. Dyer KD, et al. 2007. J. Immunol. 179:1693. (FC) PubMed
  9. Sungur CM, et al. 2013. PNAS. 110:7401. PubMed
Product Citations
  1. Wroblewska A et al. 2018. Cell. 175(4):1141-1155 . PubMed
  2. Wang Y, et al. 2021. Cancer Cell. 39:1375. PubMed
  3. McClellan BL, et al. 2022. STAR Protoc. 3:101357. PubMed
RRID
AB_2563765 (BioLegend Cat. No. 105333)

Antigen Details

Structure
Ig superfamily, 25-35 kD
Distribution

Hematopoietic stem cells and neurons, all thymocytes, peripheral T cells of the Thy-1.2 bearing mice

Function
Lymphocyte costimulation, proliferation and differentiation of hematopoietic stem cells
Ligand/Receptor
CD45
Cell Type
Hematopoietic stem and progenitors, Neurons, T cells, Thymocytes
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Craig W, et al. 1993. J. Exp. Med. 177:1331.
3. Reif AE and Schlesinger M. 1989. Cell Surface Antigen Thy-1.
4. Mayani H, et al. 1994. Blood 83:2410.

Gene ID
21838 View all products for this Gene ID
UniProt
View information about CD90.2 on UniProt.org

Related FAQs

Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?

We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.

http://techsupport.fluidigm.com/

Can I use Maxpar® Ready format clones for flow cytometry staining?

We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.

I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.

We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http://techsupport.fluidigm.com/

Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?

The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.

Other Formats

View All CD90.2 Reagents Request Custom Conjugation
Description Clone Applications
Biotin anti-mouse CD90.2 (Thy1.2) 30-H12 FC
FITC anti-mouse CD90.2 (Thy1.2) 30-H12 FC
PE anti-mouse CD90.2 (Thy1.2) 30-H12 FC
Purified anti-mouse CD90.2 (Thy1.2) 30-H12 FC,IHC-F,Costim,Depletion
APC anti-mouse CD90.2 (Thy1.2) 30-H12 FC
PE/Cyanine5 anti-mouse CD90.2 (Thy1.2) 30-H12 FC
Alexa Fluor® 488 anti-mouse CD90.2 (Thy1.2) 30-H12 FC,IHC-F
Alexa Fluor® 647 anti-mouse CD90.2 (Thy1.2) 30-H12 FC,IHC-F
Alexa Fluor® 700 anti-mouse CD90.2 (Thy1.2) 30-H12 FC
PerCP anti-mouse CD90.2 (Thy1.2) 30-H12 FC
Pacific Blue™ anti-mouse CD90.2 (Thy1.2) 30-H12 FC
PE/Cyanine7 anti-mouse CD90.2 (Thy1.2) 30-H12 FC
APC/Cyanine7 anti-mouse CD90.2 (Thy1.2) 30-H12 FC
Brilliant Violet 570™ anti-mouse CD90.2 (Thy1.2) 30-H12 FC
Brilliant Violet 785™ anti-mouse CD90.2 (Thy1.2) 30-H12 FC
Purified anti-mouse CD90.2 (Thy1.2) (Maxpar® Ready) 30-H12 FC,CyTOF®
Brilliant Violet 510™ anti-mouse CD90.2 (Thy1.2) 30-H12 FC
PerCP/Cyanine5.5 anti-mouse CD90.2 (Thy1.2) 30-H12 FC
Brilliant Violet 605™ anti-mouse CD90.2 (Thy1.2) 30-H12 FC
PE/Dazzle™ 594 anti-mouse CD90.2 (Thy1.2) 30-H12 FC
Brilliant Violet 421™ anti-mouse CD90.2 (Thy1.2) 30-H12 FC
TotalSeq™-A0075 anti-mouse CD90.2 (Thy1.2) 30-H12 PG
APC/Fire™ 750 anti-mouse CD90.2 (Thy1.2) 30-H12 FC
Brilliant Violet 711™ anti-mouse CD90.2 (Thy1.2) 30-H12 FC
Ultra-LEAF™ Purified anti-mouse CD90.2 (Thy1.2) 30-H12 FC,Depletion,Costim,IHC
TotalSeq™-C0075 anti-mouse CD90.2 (Thy1.2) 30-H12 PG
TotalSeq™-B0075 anti-mouse CD90.2 (Thy1.2) 30-H12 PG
PE/Fire™ 810 anti-mouse CD90.2 (Thy1.2) 30-H12 FC
Spark Blue™ 574 anti-mouse CD90.2 (Thy1.2) (Flexi-Fluor™) 30-H12 FC
Spark Red™ 718 anti-mouse CD90.2 (Thy1.2) (Flexi-Fluor™) 30-H12 FC
Go To Top Version: 2    Revision Date: 07/21/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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