Purified anti-mouse F4/80 Antibody

Pricing & Availability
Clone
W20065D (See other available formats)
Regulatory Status
RUO
Isotype
Rat IgG2b, κ
Ave. Rating
Submit a Review
Product Citations
publications
W20065D_PURE_F480_Antibody_051022
Thioglycolate-elicited Balb/c mouse peritoneal macrophages were stained with anti-mouse CD11b APC and purified anti-mouse F4/80 (clone W20065D) (left) or purified rat IgG2b, ĸ isotype control (right), followed by PE anti-rat IgG.
  • W20065D_PURE_F480_Antibody_051022
    Thioglycolate-elicited Balb/c mouse peritoneal macrophages were stained with anti-mouse CD11b APC and purified anti-mouse F4/80 (clone W20065D) (left) or purified rat IgG2b, ĸ isotype control (right), followed by PE anti-rat IgG.
Compare all formats
Cat # Size Price Quantity Check Availability Save
111702 100 µg £83
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

F4/80 is a 160 kD glycoprotein. It is characterized as a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80, also known as EMR1 or Ly71, has been widely used as a murine macrophage marker, which is expressed on the majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen (but not on the macrophages located in T cell areas of the spleen, lymph node and Peyer's patch), Kuffer cells, Langerhans cells, and bone marrow stromal cells. F4/80 has also been shown on a subset of dendritic cells. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for induction of CD8+ T cells-mediated peripheral tolerance.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2924451 (BioLegend Cat. No. 111702)

Antigen Details

Structure
EGF-TM7 family member, 160 kD glycoprotein
Distribution

Majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen, Kuffer cells, Langerhans cells, bone marrow stromal cells, and a subset of dendritic cells

Function
Induction of immunological tolerance
Cell Type
Macrophages, Monocytes
Biology Area
Immunology, Innate Immunity
Antigen References
  1. Austy JM and Gordon S. 1981. Eur. J. Immunol. 11:805.
  2. Hume DA, et al. 1983. J. Exp. Med. 158:1522.
  3. Ruedl C, et al. 1996. Eur. J. Immunol. 26:1801.
  4. McKnight AJ, et al. 1996. J. Biol. Chem. 271:486.
  5. Lin HH, et al. 2005. J. Exp. Med. 201:1615.
Gene ID
13733 View all products for this Gene ID
UniProt
View information about F4/80 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 05/10/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere

Login / Register
Remember me
Forgot your password? Reset password?
Create an Account