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Purified anti-mouse GPR15/BOB Antibody

Pricing & Availability
Clone
S15042I (See other available formats)
Regulatory Status
RUO
Other Names
BOB, Brother of Bonzo , G protein-coupled receptor 15, 4933439K08Rik
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
S15042I_pure_GPR15BOB_Antibody_1_122617
BALB/c splenocytes were stained with purified anti-mouse GPR15/BOB (clone S15042I) (top) or purified Rat IgG2a, κ isotype control (bottom), followed by Goat anti-rat IgG PE. The cells were then costained with CD4 APC.
  • S15042I_pure_GPR15BOB_Antibody_1_122617
    BALB/c splenocytes were stained with purified anti-mouse GPR15/BOB (clone S15042I) (top) or purified Rat IgG2a, κ isotype control (bottom), followed by Goat anti-rat IgG PE. The cells were then costained with CD4 APC.
  • S15042I_pure_GPR15BOB_Antibody_2_122617
  • S15042I_pure_GPR15BOB_Antibody_3_122617
    Mouse GPR15/BOB transfected Ba/F3 cell line was stained with purified anti-mouse GPR15/BOB (Clone S15042I) (filled histogram) or purified Rat IgG2a, κ (Isotype control) (open histogram), followed by Goat anti-rat IgG PE.
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154602 500 µg £262
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Description

GPR15, also known as BOB, is a G-protein coupled receptor with 7 transmembrane regions that is present on the cell surface as a homotrimer. Its ligand, GPR15L, was recently identified. GPR15 is expressed by effector T cells and subsets of memory T cells, and is involved in the recruitment of proinflammatory CD4+ T cells responsible of the pathogenesis in colitis. In humans, GPR15 acts as a receptor for human immunodeficiency virus (HIV) type 1 and 2, and is upregulated on monocytes and neutrophils in the synovia of patients with RA.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse GPR15-Transfectants
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Van NT, et al. 2023. Nat Commun. 7363:14. PubMed
RRID
AB_2721744 (BioLegend Cat. No. 154602)

Antigen Details

Structure
G-protein coupled receptor
Distribution

Effector and memory T cells

Function
Probable chemokine receptor, signal transduction
Cell Type
T cells, Tregs
Biology Area
Immunology
Antigen References
  1. Nguyen L, et al. 2015. Nature Immunology. 16:207
  2. Kiene M, et al. 2014. PLoS. One. 9:e88195
  3. Cartwright A, et al. 2014. Cytokine. 67:53
  4. Kim SV, et al. 2013. Science. 340:1456
  5. Okamoto Y, et al. 2013. J. Biol. Chem. 288:9189
Gene ID
71223 View all products for this Gene ID
UniProt
View information about GPR15/BOB on UniProt.org
Go To Top Version: 1    Revision Date: 12/26/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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