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Purified anti-mouse/human Galectin-3 (Mac-2) Antibody

Pricing & Availability
Clone
Gal397 (See other available formats)
Regulatory Status
RUO
Other Names
Galectin-3, Mac-2, Gal-3, RL-29, galactose-specific lectin 3, CBP-35, L-34, εBP
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
Gal397_a_Purified_Galectin-3_Mac-2_Antibody_WB_081516
Total cell lysate from MCF7 cells (lane 1, 15 µg), Jurkat cells (lane 2, 15 µg), 3T3-L1 (lane 3, 15 µg) and Raw264.7 (lane 4, 15 µg) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with purified anti-mouse/human Galectin-3 (Mac-2) antibody (clone Gal397). Proteins were visualized using an HRP Goat anti-mouse IgG Antibody and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control.
  • Gal397_a_Purified_Galectin-3_Mac-2_Antibody_WB_081516
    Total cell lysate from MCF7 cells (lane 1, 15 µg), Jurkat cells (lane 2, 15 µg), 3T3-L1 (lane 3, 15 µg) and Raw264.7 (lane 4, 15 µg) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with purified anti-mouse/human Galectin-3 (Mac-2) antibody (clone Gal397). Proteins were visualized using an HRP Goat anti-mouse IgG Antibody and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control.
  • Gal397_Pure_Galectin-3_Antibody_2_070218
    Total cell lysates (15 µg protein) from NIH3T3 (lane 1), HT29 (lane 2) and 293E (lane 3) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1 µg/mL (1:500 dilution) of purified anti-mouse/human Galectin-3 (Mac-2) antibody (clone Gal397) (upper blot). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for anti-mouse/human Galectin-3 (Mac-2) antibody or 1:5000 diluted Direct-Blot HRP anti-β-Actin antibody, clone 2F1-1(lower blot). Lane M: Molecular weight ladder.
  • Gal397_Pure_Galectin-3_Antibody_3_070218
    HeLa cells were stained with purified anti-Galectin 3 (Gal397) antibody, followed by staining with DyLight™ 594 conjugated goat anti-mouse IgG (red) antibody. Actin filaments were labeled in green. Nuclei were stained with DAPI (blue).
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126701 50 µg £49
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126702 500 µg £177
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Description

Galectin-3 (galactose-specific lectin 3) is a glycoprotein, also known as Mac-2, Gal-3, RL-29, galactose-specific lectin 3, CBP-35, L-34, and εBP. It is a member of animal β-galactoside-binding protein family that is expressed in cell nucleus, cytoplasm, plasma membrane, or extracellular matrix by variety of tumor cells, monocytes/macrophages, epithelial cells, Kupffer cells, and dendritic cells. Galectin-3 contains carbohydrate recognition domains and binds to β-galactoside residues bearing glycoproteins, such as as IgE, IgA, galactose, casein kinase I, laminin, mucin, LAMPs, and CD66. Galectin-3 is an adhesion molecule and plays an important role in regulation of cell proliferation, differentiation and tumor cell metastasis. Galectin-3 structurely possesses NWGR motif that is conserved in BH-1 domain of BCL-2 family and functions as an anti-apoptotic molecule.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Human
Reported Reactivity
African Green, Baboon, Cynomolgus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human Recombinant (partial), amino acids 151-251
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 µg per ml. For immunocytochemistry, a concentration of < 0.5 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Based on immunogen information, clone Gal397 may recognize African Green Baboon and Cynomolgus. It is recommended to test the reagent for optimal performance for other species. Due to in-house testing, the purified format is not recommended for flow cytometry and intracellular flow cytometry.

Product Citations
  1. Bülck C, et al. 2023. Sci Adv. 9:eadf4055. PubMed
  2. Pang W, et al. 2023. iScience. 26:106579. PubMed
  3. Palau V, et al. 2021. Int J Mol Sci. 23:. PubMed
  4. Palau V, et al. 2021. Int J Mol Sci. 22:. PubMed
  5. Joeh E, et al. 2021. Curr Protoc. 1:e104. PubMed
  6. Feng T, et al. 2020. EMBO Rep. 21:e50219. PubMed
  7. Du H, et al. 2021. Life Sci Alliance. 4: . PubMed
RRID
AB_1134256 (BioLegend Cat. No. 126701)
AB_1134256 (BioLegend Cat. No. 126702)

Antigen Details

Structure
Human Galetcin-3 has 250 amino acids with a predicted molecular weight of 26 kD.
Mouse Galetcin-3 has 264 amino acids with a predicted molecular weight of 27.5 kD.
Distribution

Localized in the nucleus, cytoplasm, cell surface, or extracellular matrix. Expressed by tumor cells, monocytes/macrophages, epithelial cells, Kupffer cells, dendritic cells

Function
Regulate cell proliferation and differentiation, adhesion, anti-apoptosis
Ligand/Receptor
Variety ligands, such as IgE, IgA, galactose, casein kinase I, laminin, mucin, LAMPs, CD66, etc
Cell Type
Dendritic cells, Epithelial cells, Macrophages, Monocytes
Biology Area
Cell Adhesion, Cell Biology, Cell Cycle/DNA Replication, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules
Antigen References
  1. Kim HRC, et al. 1999. Cancer Res. 59:4148
  2. Reljic R, et al. 2004. Immunol. Lett. 93:51.
  3. Takenaka Y et al. 2004. Mol. and Cell. Biol. 24:4395
  4. Joo GH, et al. 2001. J. Leukoc. Biol. 69:555
  5. Llinas L, et al. 2011. Immunol. Lett. 134:113-21
  6. Cabezón R, et al. 2011. Immunol. Lett. 134:167-73
Gene ID
16854 View all products for this Gene ID 3958 View all products for this Gene ID
UniProt
View information about Mac-2 on UniProt.org
Go To Top Version: 7    Revision Date: 08/25/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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