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Purified anti-mouse/human Helios Recombinant Antibody

Pricing & Availability
Clone
QA20A52 (See other available formats)
Regulatory Status
RUO
Other Names
IKZF2, IKAROS family zinc finger 2, ANF1A2, ZNF1A2, ZNFN1A2
Isotype
Rat IgG1, κ
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Product Citations
publications
QA20A52_PURE_Helios_RECOM_Antibody_102621
C57BL/6 mouse splenocytes were surface stained with anti-mouse CD4 APC and then fixed and perm using True-Nuclear™ Transcription Factor Buffer Set. Cells were then intracellularly stained with purified anti-Helios recombinant (clone QA20A52) (left) or rat IgG1, κ isotype control (right) followed by anti-rat IgG1 PE.
  • QA20A52_PURE_Helios_RECOM_Antibody_102621
    C57BL/6 mouse splenocytes were surface stained with anti-mouse CD4 APC and then fixed and perm using True-Nuclear™ Transcription Factor Buffer Set. Cells were then intracellularly stained with purified anti-Helios recombinant (clone QA20A52) (left) or rat IgG1, κ isotype control (right) followed by anti-rat IgG1 PE.
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105402 100 µg £182
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Description

Helios is a member of the Ikaros family of zinc finger transcription factors. It contains a C-terminal region composed of 2 zinc-finger domains that mediate dimerization between the family members. Helios was originally cloned from a mouse thymoma line. It is mainly expressed in peripheral T cells and thymocytes. It is found at high levels in a subpopulation of regulatory T cells. Helios plays an important role in T cell development and homeostasis. Overexpression of Helios profoundly alters αβ T cell differentiation and activation. It has been determined that silencing of Helios in B cells is critical for maintaining normal B cell function. Helios is also involved in tumor immunity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Human
Antibody Type
Recombinant
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICFC - Quality tested
Block - Verified
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.03 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone QA20A52 is able to completely block the staining of clone 22F6 on target cells.

RRID
AB_2904278 (BioLegend Cat. No. 105402)

Antigen Details

Structure
A zinc finger nuclear transcription factor of the Ikaros family, containing 2 zinc-finger domains in C-terminal region.
Distribution

Regulatory T cells (Tregs), conventional T cells, thymocytes

Function
Plays an important role in T cell development and homeostasis, involved in tumor immunity.
Cell Type
T cells, Thymocytes, Tregs
Biology Area
Adaptive Immunity, Cell Biology, Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References
  1. Kelly CM, et al. 1998. Curr. Biol. 8:508.
  2. Dovat S, et al. 2005. J. Immunol. 175:3508.
  3. Cortes M, et al. 1999. Curr. Opin. Immunol. 11:167.
  4. Cai Q, et al. 2009. J. Immunol. 183:2303.
  5. Zhang Z, et al. 2007. Blood 109:2190.
  6. Hahm K, et al. 1998. Genes Dev. 12:782.
Gene ID
22779 View all products for this Gene ID 22807 View all products for this Gene ID
UniProt
View information about Helios on UniProt.org
Go To Top Version: 1    Revision Date: 10/26/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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