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- Clone
- 39-10-8.RecM1 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- MHC class II
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
BALB/c mouse splenocytes were stained with anti-mouse/human CD45R/B220 (clone RA3-6B2) Brilliant Violet 421™ and purified anti-mouse I-Ad recombinant (clone 39-10-8.recM1) (left) or purified mouse IgG1, κ purified isotype control (right) followed by anti-mouse IgG1 APC.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 166902 | 100 µg | £65 | ||||
The I-Ad MHC class II alloantigen is expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2d bearing mouse strains. It is involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Recombinant
- Host Species
- Mouse
- Formulation
- 150 mM sodium chloride & 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Does not cross-react with other haplotypes (e.g., a, b, k, p, q, s).
Antigen Details
- Structure
- MHC class II
- Distribution
-
Expressed on B cell and activated T cells, antigen presenting cells of H-2d mice
- Function
- Antigen presentation
- Ligand/Receptor
- CD3/TCR, CD4
- Cell Type
- Antigen-presenting cells, B cells, T cells, Tregs
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- MHC Antigens
- Antigen References
-
- Watts C. 1997. Annu Rev Immunol. 15:821-50.
- Gene ID
- 14961 View all products for this Gene ID
Other Formats
View All I-Ad Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-mouse I-Ad Recombinant Antibody | 39-10-8.RecM1 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse I-Ad Recombinant Antibody
BALB/c mouse splenocytes were stained with anti-mouse/human ...
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