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Purified anti-mouse I-Ak (Aαk) Antibody

Pricing & Availability
Clone
11-5.2 (See other available formats)
Regulatory Status
RUO
Other Names
MHC class II
Isotype
Mouse (BALB/c) IgG2b, κ
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Product Citations
publications
11-5dot2_Pure_071108.jpg
C3H mouse splenocytes stained with 11-5.2 purified, followed by anti-mouse IgG2b FITC
  • 11-5dot2_Pure_071108.jpg
    C3H mouse splenocytes stained with 11-5.2 purified, followed by anti-mouse IgG2b FITC
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110002 500 µg £174
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Description

The 11-5.2 antibody reacts with the α chain of the I-Ak MHC class II alloantigen. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2k bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The 11-5.2 antibody cross-reacts with cells from mice bearing the H-2r haplotype. Reactivity with other H-2 haplotypes (e.g., b, d, f, p, q, s) has not been observed. The 11-5.2 antibody has been reported to block antigen presentation and induce B cell differentiation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
CKB mouse splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F, IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, blocking of MHC class II - restricted CD4+ T cell responses2,3, induction of B cell differentiation4, and immunohistochemical staining5 of acetone-fixed frozen sections.

This clone does not cross react with other haplotypes (e.g., b, d, f, p, q, s).

Application References

(PubMed link indicates BioLegend citation)
  1. Oi VT, et al. 1978. Curr. Top. Microbiol. Immunol. 81:115. (IP)
  2. Formby B, et al. 1990. P. Natl. Acad. Sci. USA 87:2438.
  3. Kappler JW, et al. 1981. J. Exp. Med. 153:1198.
  4. Bishop GA, et al. 1989. P. Natl. Acad. Sci. USA 86:5933.
  5. Ardehali A, et al. 1995. Circulation 92:450. (IHC)
  6. Turnquist HR, et al. 2007. J. Immunol. 178:7018.
  7. Zhang L, et al. 2011. Lupus. 20:667. PubMed.
Product Citations
  1. Zhang L, et al. 2011. Lupus. 1.296527778. PubMed
  2. Hausmann A, et al. 2021. J Exp Med. 218:. PubMed
  3. Barroso M, et al. 2015. J Biol Chem. 290: 27101 - 27112. PubMed
RRID
AB_313459 (BioLegend Cat. No. 110002)

Antigen Details

Structure
α chain of MHC class II
Distribution

B cell and activated T cells, APCs of H-2b mice

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD4
Cell Type
Antigen-presenting cells, B cells, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.

Gene ID
14960 View all products for this Gene ID
UniProt
View information about I-Ak on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 08/11/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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