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Purified anti-mouse IFNAR-1 Antibody

Pricing & Availability
Clone
MAR1-5A3 (See other available formats)
Regulatory Status
RUO
Other Names
IFNAR1, IFN-αR1, IFN-α/β Receptor Subunit 1
Isotype
Mouse IgG1, κ
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Product Citations
publications
MAR1-5A3_Pure_011909.jpg
C57BL/6 splenocytes stained with purified MAR1-5A3, followed by biotinylated anti-mouse IgG1 and Sav-PE
  • MAR1-5A3_Pure_011909.jpg
    C57BL/6 splenocytes stained with purified MAR1-5A3, followed by biotinylated anti-mouse IgG1 and Sav-PE
Compare all formats
Cat # Size Price Quantity Check Availability Save
127301 25 µg £49
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127302 100 µg £73
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Description

IFNAR-1, the type I IFN receptor subunit 1, is coexpressed with IFNAR-2 on nearly all cells and make up the heterodimeric receptor complex that binds to all type I IFNs (IFN-α/β). Type I IFNs are a group of structurally and functionally related cytokines that have been shown to promote anti-viral, anti-microbial, anti-tumor, and autoimmune responses. Ligand binding to the IFN-α/β receptor complex leads to the tyrosine phosphorylation and activation of IFNAR-1-associated Tyk2 and IFNAR-2-associated Jak1 signal transductions.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Exogenously expressed murine IFNAR1 extracellular domain
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IP, WB, ELISA, Block - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: Western blotting1,2, immunoprecipitation1, ELISA1, and blocking8,9. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 127322) (Endotoxin <0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Sheehan KC, et al. 2006. J. Interferon Cytokine Res. 26:804. (FC, Block, IP, WB, ELISA)
  2. Dunn GP, et al. 2005. Nat. Immunol. 6:722. (FC, WB)
  3. Miller JC, et al. 2008. J. Immunol. 181:8492. PubMed
  4. Habjan M, et al. 2009. J. Virol. 83:4365. PubMed
  5. Kelly-Scumpia KM, et al. 2010. J. Exp. Med. 207:319. PubMed
  6. Swanson CL, et al. 2010. J. Exp. Med. 207:1485. PubMed
  7. Marshall HD, et al. 2011. J Virol. epub. PubMed
  8. Swann JB, et al. 2007. J. Immunol. 178:7540. (Block)
  9. Jones GW, et al. 2011. FASEB J. 25:409. (Block)
  10. Mitzel DN, et al. 2014. J. Immunol. 192:4273. PubMed
Product Citations
  1. Potempa M, et al. 2022. J Immunol. 208:1742. PubMed
  2. Swanson C, et al. 2010. J Exp Med. 207:1485. PubMed
  3. Wang J, et al. 2019. BMC Infect Dis. 19:1031. PubMed
  4. Hou J, et al. 2021. Nat Commun. 12:2970. PubMed
  5. Guo P, et al. 2021. J Immunol. 207:408. PubMed
  6. Borriello F, et al. 2018. Front Immunol. 8:1772. PubMed
  7. Chowdhury D, et al. 2021. Front Immunol. 12:755961. PubMed
  8. Mitzel D, et al. 2014. J Immunol. 192:4273. PubMed
  9. Kelly-Scumpia K, et al. 2010. J Exp Med. 207:319. PubMed
  10. Marshall H, et al. 2011. J Virol. 85:5929. PubMed
  11. Habjan M, et al. 2009. J Virol. 83:4365. PubMed
  12. Sun J, et al. 2011. Biochem Pharmacol. 82:1175. PubMed
  13. Van Winkle JA, et al. 2020. J Virol. 94:. PubMed
RRID
AB_1089153 (BioLegend Cat. No. 127301)
AB_1089153 (BioLegend Cat. No. 127302)

Antigen Details

Distribution

IFNAR1 and IFNAR2 are coexpressed on nearly all cells.

Ligand/Receptor
IFN-α/β
Antigen References

1. Branca AA, et al. 1981. Nature 294:768.
2. Orchansky P, et al. 1984. J. Interferon Res. 4:275.
3. Hemmi S, et al. 1994. Cell 76:803.
4. Novick D, et al. 1994. Cell 77:391.

Gene ID
15975 View all products for this Gene ID
UniProt
View information about IFNAR-1 on UniProt.org

Other Formats

View All IFNAR-1 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-mouse IFNAR-1 MAR1-5A3 FC,IP,WB,ELISA,Block
Biotin anti-mouse IFNAR-1 MAR1-5A3 FC,IP,WB,ELISA
PE anti-mouse IFNAR-1 MAR1-5A3 FC
APC anti-mouse IFNAR-1 MAR1-5A3 FC
Ultra-LEAF™ Purified anti-mouse IFNAR-1 MAR1-5A3 FC,IP,WB,ELISA,Block
PE/Cyanine7 anti-mouse IFNAR-1 MAR1-5A3 FC
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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