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Purified anti-mouse IL-10 Antibody

Pricing & Availability
Clone
JES5-2A5 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-10, Cytokine synthesis inhibitory factor (CSIF), B cell derived T cell growth factor (B-TCGF), T cell growth inhibitory factor (TGIF)
Isotype
Rat IgG1, κ
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Product Citations
publications
JES5-16E3
  • JES5-16E3
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Cat # Size Price Quantity Check Availability Save
504901 50 µg £57
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504902 500 µg £97
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Description

IL-10 was originally described as Cytokine Synthesis Inhibitory Factor (CSIF) by virtue of its ability to inhibit cytokine production by Th1 clones. IL-10 shares over 80% sequence homology with the Epstein-Barr virus protein BCRFI. The biological activities of IL-10 include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed type hypersensitivity response, and stimulation of the Th2 cell response, which results in elevated antibody production. The JES5-2A5 antibody reacts with mouse interleukin-10 (IL-10). The JES5-2A5 antibody can neutralize the bioactivity of natural or recombinant IL-10.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
E. coli-expressed, recombinant mouse IL-10
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture, WB
Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 4-8 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-10 recombinant protein ranging from 2000 to 15 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
* For ELISA capture, it is very critical to use 0.2 M Sodium Phosphate Buffer, pH 6.5 (11.8g Na2HPO4, 16.1g NaH2PO4; q.s. to 1.0 L) as coating buffer.
Note: Carbonate buffer, pH 9.5 should not be used as coating buffer for JES5-2A5. It may cause high background and lower sensitivity.

Application Notes

ELISA or ELISPOT Capture1-5,7,10: The purified JES5-2A5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated JES5-16E3 antibody (Cat. No. 505004) as the detecting antibody. The LEAF™ purified antibody is suggested for ELISPOT capture.

ELISA or ELISPOT Detection: The biotinylated JES5-2A5 antibody is useful as the detecting antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the Purified JES5-16E3 antibody (Cat. No. 505002/505012) as the capture antibody.

Neutralization2,8,9: The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse IL-10 bioactivity in vivo and in vitro (Cat. No. 504904).

Additional reported applications (for the relevant formats) include: Western blotting12 and in vivo capture6.

Note: For testing mouse IL-10 in serum, plasma or supernatant, BioLegend's ELISA Max™ Sets (Cat. No. 431401 to 431406) are specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Sander B, et al. 1993. J. Immunol. Meth. 166:201.
  2. Abrams J, et al. 1992. Immunol. Rev. 127:5.
  3. Abrams J, 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.20.
  4. Mo X, et al. 1995. J. Virol. 69:1288.
  5. Sarawar S, et al. 1994. J. Immunol. 153:1246.
  6. Finkelman F, et al. 2003. Curr. Prot. Immunol. John Wiley & Sons, New York. Unit 6.28.
  7. Brummel R and Lenert P, 2005. J. Immunol. 174:2429.
  8. Hara M, et al. 2001. J. Immunol. 166:3789.
  9. Riemann M, et al. 2005. J. Immunol. 175:3560.
  10. Xu G, et al. 2007. J. Immunol. 179:5358.
  11. Guo Z, et al. 2013. J. Immunol. 190:4337. PubMed
  12. van der Poll T, et al. 1995. J. Immunol. 155:5397. (WB)
Product Citations
  1. Min CK, et al. 2018. Front Immunol. 1.779166667. PubMed
  2. Maerz JK, et al. 2020. Front Immunol. 10:3093. PubMed
  3. Campos–Mora M, et al. 2019. Front Immunol. 1.029166667. PubMed
  4. Yan J, et al. 2020. Cell Rep. 107820:31. PubMed
  5. Di Conza G, et al. 2021. Nat Immunol. 22:1403. PubMed
  6. Ranjan K, et al. 2021. J Clin Invest. 131:. PubMed
  7. Xu G, et al. 2007. J Immunol. 179:5358. PubMed
  8. Riemann M, et al. 2005. J Immunol. 175:3560. PubMed
  9. He L, et al. 2018. Immunology. 153:84. PubMed
RRID
AB_315349 (BioLegend Cat. No. 504901)
AB_315349 (BioLegend Cat. No. 504902)

Antigen Details

Structure
Acid-labile cytokine; dimer; 17-21 kD (Mammalian)
Bioactivity
Inhibits IFN-γ, TNF-β, IL-2 production by TH1 clones; inhibits macrophage-mediated IL-1, IL-6, TNF-α synthesis; suppresses delayed type hypersensitivity response; stimulates TH2 cell response; mast cell proliferation
Cell Sources
Activated CD8+ T cells, TH0, TH2 subset of CD4+ T cells, Ly-1+ B cells, monocytes, macrophages, keratinocytes
Cell Targets
T cells, B cells, mast cells, macrophages
Receptors
IL-10R (CDw210)
Cell Type
Tregs
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. de Waal-Malefy, T, et al. 1992. Curr. Opin. Immunol. 4:314.
3. Howard, M., et al. 1992. Immunol. Today. 13:198.
4. Quesniaux, V. 1992. Research Immunol. 143:385.

Regulation
Downregulated by IL-4, IL-10
Gene ID
16153 View all products for this Gene ID
UniProt
View information about IL-10 on UniProt.org

Other Formats

View All IL-10 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-mouse IL-10 JES5-2A5 ELISA Capture,WB
Biotin anti-mouse IL-10 JES5-2A5 ELISA Detection,ELISPOT Detection,WB
Ultra-LEAF™ Purified anti-mouse IL-10 JES5-2A5 ELISA Capture,ELISPOT Capture,Neut,WB
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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  • Ultra-LEAF™ Purified anti-mouse IL-10

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