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Purified anti-mouse IL-12 (p70) Antibody

Pricing & Availability
Clone
C18.2 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-12 p70, Cytotoxic lymphocyte maturation factor (CLMF), Natural killer cell stimulatory factor (NKSF)
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
IL-12_p70_Pair_061608.jpg
  • IL-12_p70_Pair_061608.jpg
Cat # Size Price Quantity Check Availability Save
511801 50 µg £65
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511802 500 µg £209
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Description

Interleukin 12 (IL-12) is a heterodimeric pleiotropic 70 kD (p70) cytokine consisting of a 40 kD (p40) subunit and a 35 kD (p35) subunit. IL-12 is a potent regulator of cell-mediated immune responses and key mediator of Th1 cell development. Bioactive IL-12 is secreted by activated B lymphocytes, dendritic cells, and macrophages in response to pathogens or upon interaction with activated T cells.IL-12 p70 stimulates cytotoxic T, NK and LAK cell proliferation, andinduces IFN-γ and TNF production in resting and activated T and NK cells.It has been suggested that IL-12 may have therapeutic potential as a vaccine adjuvant that promotes cellular-immunity and as an anti-tumor and anti-viral agent.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
CHO expressed, recombinant mouse IL-12 p70
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture
Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA capture antibody, a concentration range of 1-4 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-12(p70) recombinant protein ranging from 500 to 4 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Capture: The purified C18.2 antibody is useful as a capture antibody for a sandwich ELISA assay, when used in conjunction with the biotinylated C17.8 antibody as detection antibody and recombinant mouse IL-12 p70 as the standard.

Product Citations
  1. Volpedo G, et al. 2022. NPJ Vaccines. 7:32. PubMed
  2. Liang D, et al. 2018. Front Immunol. 8:1801. PubMed
  3. Zhan CS, et al. 2020. FASEB J. 14006:34. PubMed
  4. Jin X, et al. 2020. Cell Reports. 31(8):107690. PubMed
  5. Zhou Y, et al. 2018. Cell Mol Immunol. 15:246. PubMed
  6. Liu C, et al. 2019. Theranostics. 9:1369. PubMed
  7. Zhao L, et al. 2021. STAR Protocols. 2:100732. PubMed
RRID
AB_2123771 (BioLegend Cat. No. 511801)
AB_2123771 (BioLegend Cat. No. 511802)

Antigen Details

Structure
heterodimeric cytokine composed of p40 subunit shared with IL-23 and a specific p35 subunit
Distribution
Secreted by dendritic cells, monocytes/macrophages, B cells, T cells
Function
Downregulated by IL-10; homodimeric p40 antagonistic to functional p70 heterodimer; p40 monomer has no function; p40 subunit in common with IL-23
Interaction
T cells, NK cells
Ligand/Receptor
IL-12Rβ1 binds p40 shared with IL-23; dimeric with IL-12Rβ2 binds p35
Bioactivity
IL-12 p70 (p40:p35) induces IFN-γ, TNF-α production in T and NK cells; costimulation of PBL proliferation; proliferation/differentiation of TH1 T lymphocytes.
Cell Type
Dendritic cells, Monocytes, Macrophages, B cells, T cells
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Lankford CS, et al. 2003 J. Leukoc. Biol.. 73:49.
2. Parham C, et al. 2002 J. Immunol.. 168:5699.
3. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
4. Trinchieri G, et al. 1993 Immunol. Today. 14:335.
5. Quesniaux V. 1992. Research Immunol. 143:385.
6. Trinchieri G, et al. 1992 Prog. Growth Factor Res. 4:355.

Gene ID
16159 View all products for this Gene ID 16160 View all products for this Gene ID
UniProt
View information about IL-12 p70 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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  • Purified anti-mouse IL-12 (p70)

    IL-12_p70_Pair_061608.jpg
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