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Purified anti-mouse IL-7 Antibody

Pricing & Availability
Clone
A18052C (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-7, Lymphopoietin (LP-1), Pre-B cell growth factor, Thymocyte growth factor
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A18052C_PURE_-IL-7-_Direct-ELISA_Antibody
Direct ELISA showing purified anti-mouse IL-7 (clone A18052C) bound to plate-immobilized recombinant mouse IL-7 protein (Cat Nos. 577802, 577804, 577806, and 577808). The wells were incubated with serially diluted primary antibody at room temperature for 2 hours in 1% BSA-PBS, followed by incubation with HRP Goat anti-rat IgG (Cat. No. 405405). TMB High Sensitivity Substrate Solution (Cat. No. 421501) was used for detection. Absorbance was measured at 450 nm.
  • A18052C_PURE_-IL-7-_Direct-ELISA_Antibody
    Direct ELISA showing purified anti-mouse IL-7 (clone A18052C) bound to plate-immobilized recombinant mouse IL-7 protein (Cat Nos. 577802, 577804, 577806, and 577808). The wells were incubated with serially diluted primary antibody at room temperature for 2 hours in 1% BSA-PBS, followed by incubation with HRP Goat anti-rat IgG (Cat. No. 405405). TMB High Sensitivity Substrate Solution (Cat. No. 421501) was used for detection. Absorbance was measured at 450 nm.
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630452 100 µg £284
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Description

Mouse IL-7 was initially described as a pre-B-cell grow factor expressed in bone marrow stromal cells. IL-7 is essential for normal murine B cell development, and plays a key role in regulating the homeostasis and function of the T-cells. The binding of IL-7 to its receptor induces dimerization of IL-7Ra and the common gamma chain (gc) which leads to activation of receptor associated tyrosine Janus kinases JAK1 (IL-7R) and JAK3 (γc). The activated JAK proteins in turn phosphorylate specific residues on the IL-7R creating docking sites for signaling molecules such as STAT5 and to a lesser extent STAT1 and STAT3. In adult mice, IL-7/IL-7R signaling up regulates expression of early B cell factor (EBF), and EBF in turn regulates expression of B cell-specific genes required for the transition from lymphoid progenitor to pro-B cells. Besides, IL-7 plays a role in the development of secondary lymphoid tissues. IL-7 is necessary to specify CD8 lineage differentiation during CD4/CD8 cell fate choice in the thymus by inducing expression of the transcription factor Runx3. IL-7 induces antiapoptotic factors Bcl2 and Bcl-xL and inhibiting pro-apoptotic factors such as Bad and Bax. In this fashion, IL-7 induces cell activation, survival, and proliferation of T lymphocytes. In addition, IL-7 controls T-cell size and metabolism through the activation of PI3 kinase-dependent pathways and regulation of glucose metabolism. IL-7 also controls T cell–dendritic cell interactions that are essential for both T-cell homeostasis and activation in vivo. CD4 T cell lymphopenia increases the expression of circulating IL-7, and TGFb induces IL-7 downregulation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse Recombinant IL-7 Protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

Direct ELISA - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Direct ELISA. The optimal concentration of this reagent for the specific application and the experiment design should be established by the end-user

Additional Product Notes

This product is intended for sandwich-based immunoassay development on various assay platforms requiring antibody pairs. This antibody (clone A18052C) could function as a capture antibody when paired with biotin anti-mouse IL-7 antibodies (Cat. No. 630253) (clone A18052H) or (Cat. No. 625353) (clone Poly22A09) as the detection antibodies and using Recombinant Mouse IL-7 protein (Cat Nos. 577802, 577804, 577806, and 577808) as the assay standard. For assay development flexibility purposes, this product is provided as a purified format. 

RRID
AB_3097287 (BioLegend Cat. No. 630452)

Antigen Details

Structure
Cytokine
Distribution

Thymic and bone morrow stromal cells, keratinocytes, dendritic cells, endothelial cells, intestinal epithelial, and epithelial goblet cells

Function
IL-7 is essential for B cell production
Interaction
Progenitor B and T cells, CD4-CD8- thymocytes, naïve and memory CD4 and CD8 T cells, and megakaryocytes
Ligand/Receptor
Heterodimer IL-7Rα (CD127); γ-subunit (CD132) which shares component with IL-2, IL-4, IL-13, IL-15, and IL-21 receptors
Cell Type
Embryonic Stem Cells
Biology Area
Cancer Biomarkers, Neuroinflammation, Neuroscience, Stem Cells
Antigen References
  1. Yokota T, et al. 1986. P Natl Acad Sci USA. 83:5894.
  2. Meier D, et al. 2007. Immunity. 26:643-54.
  3. Parish JK, et al. 2009. J Immunol. 182:4255-66.
  4. Saini M, et al. 2009. Blood. 113:5793-800.
  5. Park JH, et al. 2010. Nat Immunol. 11:257-64.
  6. Li WQ, et al. 2010. Mol Cell Biol. 30:590-600.
Gene ID
16196 View all products for this Gene ID
UniProt
View information about IL-7 on UniProt.org
Go To Top Version: 1    Revision Date: 12/06/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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