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Purified anti-mouse IRF5 Antibody

Pricing & Availability
Clone
W16007B (See other available formats)
Regulatory Status
RUO
Other Names
IRF5, Irf5, IRF-5, Interferon regulatory factor 5
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W16007B_PURE_IRF5_Antibody_1_081920
Whole cell extracts (15 µg protein) from C2C12 (reduced expression control), RAW264.7 (positive control), and THP-1 (mouse reactivity control) cells were resolved on a 4-12% Bis-Tris gel, transferred to PVDF and probed with 0.1 µg/mL (1:5000 dilution) of purified anti-IRF5 antibody (clone W16007B) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin antibody (Cat. No. 643807) was used as a loading control at a 1:20000 dilution (lower). Lane M: Molecular weight marker.
  • W16007B_PURE_IRF5_Antibody_1_081920
    Whole cell extracts (15 µg protein) from C2C12 (reduced expression control), RAW264.7 (positive control), and THP-1 (mouse reactivity control) cells were resolved on a 4-12% Bis-Tris gel, transferred to PVDF and probed with 0.1 µg/mL (1:5000 dilution) of purified anti-IRF5 antibody (clone W16007B) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin antibody (Cat. No. 643807) was used as a loading control at a 1:20000 dilution (lower). Lane M: Molecular weight marker.
  • W16007B_PURE_IRF5_Antibody_2_081920
    C57BL/6 mouse splenocytes were fixed, permeabilized, and then stained with purified anti-mouse IRF5 antibody (clone W16007B) (left) or purified rat IgG2a, κ isotype control (right) followed by polyclonal goat anti-rat IgG secondary antibody PE. Cells were then washed and stained with anti-mouse/human B220 APC.
  • W16007B_PURE_IRF5_Antibody_3_081920
    Whole cell extracts (250 µg total protein) prepared from RAW264.7 cells were immunoprecipitated overnight with 2.5 µg of purified rat IgG2a, κ isotype ctrl antibody (Cat. No. 400502) or purified anti-IRF5 antibody (clone W16007B). The resulting IP fractions and whole cell extract input (6%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with clone W16007B. Lane M: Molecular weight marker.
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158601 25 µg £73
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158602 100 µg £238
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Description

The interferon regulatory factor (IRF) family of transcription factors comprises nine members with conserved domains. IRF5 mediates the induction of proinflammatory cytokine, such as IL-6 and TNFa. IRF5 is highly expressed in monocytes, macrophages, B cells, and dendritic cells. Expression of IRF5 is associated with many autoimmune diseases.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse IRF5 recombinant protein (149-400 a.a.)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICFC, IP - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.05 - 0.1 µg/mL. For intracellular flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone was tested for ICC using RAW264.7 cells fixed with 4% PFA and permeabilized with either Triton X-100 or methanol. While both methods were compatible with IRF5 staining, the signal was weak and did not meet our sensitivity standards.

This clone does not recognize human IRF5. For detection of human IRF5, please use BioLegend clone 11F4A09 (Cat. No. 652602).

During product development testing of this clone for western blot, a ~37 kD protein of unknown origin was faintly detected. This protein may represent a minor IRF5 degradation product.

RRID
AB_2904302 (BioLegend Cat. No. 158601)
AB_2904302 (BioLegend Cat. No. 158602)

Antigen Details

Structure
497 a.a. (56 kD)
Distribution

Monocytes, macrophages, B cells, dendritic cells

Function
Involved in IFNa and IFNb induction in response to viral infection
Cell Type
B cells, Macrophages, Monocytes
Biology Area
Immunology, Signal Transduction
Molecular Family
Adaptor Proteins, Immune Checkpoint Receptors
Antigen References
  1. Kobayashi S, et al. 2019. J Immunol. 203(6):1447-1456.
  2. Nie S, et al. 2019. Clin Immunol. 207:24-35.
  3. Pandey SP, et al. 2019. Mucosal Immunol. 12(4):1065.
Gene ID
27056 View all products for this Gene ID
UniProt
View information about IRF5 on UniProt.org
Go To Top Version: 1    Revision Date: 08/19/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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