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Purified anti-mouse Isthmin-1 Antibody

Pricing & Availability
Clone
B054B6 (See other available formats)
Regulatory Status
RUO
Other Names
Ism1, BATAM
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
B054B6_Purified_Isthmin-1 _Antibody_ICFC_101712
Mouse Isthmin-1 transfected HEK-293E cells were fixed, permeabilized, and then stained with purified Isthmin-1 (clone B054B6, filled histogram) or purified rat IgG2a, κ isotype control (open histogram), followed by anti-rat IgG FITC.
  • B054B6_Purified_Isthmin-1 _Antibody_ICFC_101712
    Mouse Isthmin-1 transfected HEK-293E cells were fixed, permeabilized, and then stained with purified Isthmin-1 (clone B054B6, filled histogram) or purified rat IgG2a, κ isotype control (open histogram), followed by anti-rat IgG FITC.
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145001 25 µg £101
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Description

Isthmin is a secreted protein, and it was initially identified in the Xenopus midbrain-hindbrain organizer (MHB) or isthmus organizer, where it is highly expressed. The MHB is an important signaling center in vertebrates and regulates the polarized morphological differentiation of the adjacent tectum and cerebellum. Mouse Isthmin is a 454 amino acid protein containing a Thrombospondin Type 1 Repeat (TSR) domain in the central region and an Adhesion-associated domain in MUC4 and Other Proteins (AMOP) domain at the C-terminal. The TSR domains are highly conserved with 98% identity between mouse and human; 87-88% identity between mouse and Xenopus. The C-terminal AMOP domains are also highly conserved with 99% identity between mouse and human; 91% identity between mouse and Xenopus. Mouse isthmin nucleotide sequence is similar to the human chromosome 20 open reading frame 82 (C20orf82). The Ism1 gene is conserved in human, chimpanzee, dog, cow, chicken, and zebrafish. Isthmin has angiostatic activity in vitro and in vivo. Isthmin inhibits EC capillary network formation mainly by interfering with the early stages of in vitro angiogenesis on matrigel. Isthmin inhibits VEGF-induced EC proliferation and induces apoptosis in the presence of VEGF. Overexpression of isthmin in B16 melanoma inhibits tumor growth and tumor angiogenesis in mice. Knockdown of isthmin in zebrafish embryos leads to abnormal intersegmental vessel (ISV) formation in the trunk.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Full length mouse Isthmin 1 recombinant protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2561677 (BioLegend Cat. No. 145001)

Antigen Details

Structure
454 aa with a predicted molecular weight of 51 kD.
Distribution

Isthmin is highly expressed in the midbrain-hindbrain organizer.

Interaction
Isthmin interacts with endothelial cells.
Biology Area
Cell Biology, Signal Transduction, Ubiquitin/Protein Degradation
Antigen References

1. Pera EM, et al. 2002. Mech. Dev. 116:169.
2. Xiang W, et al. 2009. J. Cell. Mol. Med. 15:359.
3. Zhang Y, et al. 2011. Cell Death. Dis. 2:e153.

Gene ID
319909 View all products for this Gene ID
UniProt
View information about Isthmin-1 on UniProt.org
Go To Top Version: 2    Revision Date: 08/22/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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