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Purified anti-mouse Ki-67 Recombinant Antibody

Pricing & Availability
Clone
QA18A32 (See other available formats)
Regulatory Status
RUO
Other Names
Mki67, Ki67, Ki-67, MIB-1, KIA
Isotype
Rat IgG1, κ
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Product Citations
publications
1_QA18A32_PURE_Ki-67_RECOM_IHC_102221
C57BL/6 mouse frozen intestine section was fixed with 4% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for ten minutes, and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/mL of purified anti-mouse Ki-67 (clone QA18A32) overnight at 4°C, followed by 2.5 µg/mL of anti-rat IgG (clone Poly4054) Alexa Fluor® 647 (red) for two hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured by 10X objective.
  • 1_QA18A32_PURE_Ki-67_RECOM_IHC_102221
    C57BL/6 mouse frozen intestine section was fixed with 4% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for ten minutes, and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/mL of purified anti-mouse Ki-67 (clone QA18A32) overnight at 4°C, followed by 2.5 µg/mL of anti-rat IgG (clone Poly4054) Alexa Fluor® 647 (red) for two hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured by 10X objective.
  • 2_QA18A32_PURE_Ki-67_RECOM_ICC_102221
    TE‐71 cells were fixed with 1% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5% Triton X‐100 for ten minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 2.5 μg/mL of purified anti-mouse Ki-67 (clone QA18A32) overnight at 4°C, followed by 2.5 µg/mL of anti-rat IgG (clone Poly4054) Alexa Fluor® 647 (red) for two hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured by 40X objective.
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163202 100 µg £94
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Description

The nuclear protein Ki-67 was first identified by the monoclonal antibody Ki-67, which was generated by immunizing mice with nuclei of the L428 Hodgkin lymphoma cell line. Ki-67 protein plays an essential role in ribosomal RNA transcription and cell proliferation. Expression of Ki-67 occurs during G1, S, G2, and M phase. While in G0 phase, the Ki-67 protein is not detectable. Ki-67 is strongly expressed in proliferating cells and has been reported as a prognostic marker in various tumors.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Recombinant
Host Species
Rat
Immunogen
E. coli expressed, N-terminal His-Thioredoxin-tagged, partial mKi-67 (1816-2163 aa) recombinant protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-F - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2904309 (BioLegend Cat. No. 163202)

Antigen Details

Structure
325 kD protein containing a forkhead-associated (FHA) domain and 13 tandem repeats.
Distribution

Nucleus and chromosomes

Function
Required for cell cycle progression and proliferation.
Interaction
Interacts with KIF15; binds to MKI67IP through FHA domain
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Immunology
Antigen References
  1. Starborg M, et al. 1996. J. Cell. Sci. 109:143.
  2. Byeon IJ, et al. 2005. Nat. Struct. Mol. Biol. 12:987.
  3. Yerushalmi R, et al. 2010. Lancet. Oncol. 11:174.
  4. Beltrami AP, et al. 2001. N. Engl. J. Med. 344:1750.
  5. Sachsenberg N, et al. 1998. J. Exp. Med. 187:1295.
  6. Nagy Z, et al. 1997. Acta. Neuropathol. 93:294.
Gene ID
4288 View all products for this Gene ID
UniProt
View information about Ki-67 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 10/22/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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