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Purified anti-mouse KLRG1 (MAFA) Recombinant Antibody

Pricing & Availability
Clone
QA20A64 (See other available formats)
Regulatory Status
RUO
Other Names
Killer cell lectin-like receptor G1 (KLRG1), mast cell function-associated antigen (MAFA or 2F1-Ag)
Isotype
Rat IgG1, κ
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Product Citations
publications
QA20A64_PURE_KLRG1_Antibody_122821
C57BL/6 mouse splenocytes stained with anti-mouse NK-1.1 APC and purified anti-mouse KLRG1 recombinant (clone QA20A64) (left) or purified rat IgG1, κ isotype control (right), followed by anti-rat IgG1 PE.
  • QA20A64_PURE_KLRG1_Antibody_122821
    C57BL/6 mouse splenocytes stained with anti-mouse NK-1.1 APC and purified anti-mouse KLRG1 recombinant (clone QA20A64) (left) or purified rat IgG1, κ isotype control (right), followed by anti-rat IgG1 PE.
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164902 100 µg £273
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Description

Killer cell lectin-like receptor G1 (KLRG1) is the mouse homolog of the rat mast cell function-associated antigen (MAFA or 2F1-Ag). KLRG1 is a type II membrane glycoprotein that was first identified on the surface of rat mast cell line RBL-2H3. It is composed of a homodimer of glycosylated 30-38 kD subunits. Mouse and human homologs of KLRG1 are expressed by subsets of NK cells and lymphokine-activated killer (LAK) cells but not mast cells.  KLRG1 is also expressed on subsets of CD8+ and CD4+ cells, including CD4+ and CD8+ effector/memory cells, potent regulatory CD4+ T cells. KLRG1 may be involved in regulating NK cell homeostasis. KLRG1 was found to recognize cadherins and thus inhibit immune responses by regulating the effector function and the developmental processes of NK and T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Recombinant
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone QA20A64 completely block the binding of reported clone 2F1/KLRG1 on target cells.

RRID
AB_2910348 (BioLegend Cat. No. 164902)

Antigen Details

Structure
KLRG1 is an inhibitory lectin-like type II transmembrane receptor containing a cytoplasmic motif similar to ITIM, expressed mainly as a homodimeric molecule consisting of two N-glycosylated subunits of approximately 30-38 kD.
Distribution

Subsets of NK cells, lymphokine-activated killer (LAK) cells and subsets of CD8+ and CD4+ cells.

Function
Regulates NK cell proliferation, maturation, and homeostasis. Inhibits immune responses by regulating the effector function and the developmental processes of NK and T cells.
Interaction
NK cells, T cells, Tregs
Ligand/Receptor
E-cadherin
Cell Type
NK cells, T cells
Biology Area
Immunology, Innate Immunity
Antigen References
  1. Grundemann C, et al. 2006. J. Immunol. 176:1311-5.
  2. Blaser C, et al. 1998. J. Immunol. 161:6451-4.
  3. Huntington ND, et al. 2007. J. Immunol. 178:4764-70.
  4. Voehringer D, et al. 2001. J. Immunol. 167:4838-43.
Gene ID
10219 View all products for this Gene ID
UniProt
View information about KLRG1 on UniProt.org
Go To Top Version: 1    Revision Date: 12/28/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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