Purified anti-mouse Ly-6A/E (Sca-1) Recombinant Antibody, Ly-6A/E, QA17A36

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Clone: QA17A36 (See other available formats)
Regulatory Status: RUO
Other Names: Sca-1
Isotype: Rat IgG1, κ
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Product Citations: publications

C57BL/6 mouse splenocytes were stained with purified Ly-6A/E (clone QA17A36, filled histogram) or purified rat IgG1, κ isotype control (open histogram), followed by anti-rat IgG1 PE Antibody.

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163802

100 µg

£70

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Description

Ly-6A/E, also known as Sca-1, is an 18 kD member of the Ly-6 multigene family. Ly6A/E is a glycosylphosphatidylinositol (GPI)-linked protein expressed on hematopoietic stem cells. In mice expressing the Ly-6.2 haplotype (e.g., AKR, C57BL, C57BR, DBA/2, SJL, SWR, and 129), Ly-6A/E is also expressed on peripheral B lymphocytes and thymic and peripheral T lymphocytes. Strains expressing the Ly-6.1 haplotype (e.g., BALB/c, CBA, C3H/He, DBA/1, and NZB) have low Ly-6A/E expression on resting peripheral lymphocytes. The expression of Ly-6A/E on lymphocytes is upregulated upon activation from both Ly6.1 and Ly6.2 haplotype mice. Ly-6A/E is thought to be involved in the regulation of both T and B cell responses.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Antibody Type: Recombinant
Host Species: Rat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2910344 (BioLegend Cat. No. 163802)

Antigen Details

Structure: Ly-6 multigene family, 18 kD
Distribution: Hematopoietic stem cells, activated T cells and B cells, subset of resting B cells and T cells
Function: Regulates B and T cell responses
Antigen References: Upadhyay G. 2019. Front Immunol. 10:819.

Morrison SJ, et al. 1994. Immunity. 1:661.

Spangrude GJ, et al. 1988. J Immunol. 141:3697.
Gene ID: 110454 View all products for this Gene ID
UniProt: View information about Ly-6A/E on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Other Formats

View All Ly-6A/E Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-mouse Ly-6A/E (Sca-1) Recombinant Antibody	QA17A36	FC
PE anti-mouse Ly-6A/E (Sca-1) Recombinant Antibody	QA17A36	FC
APC anti-mouse Ly-6A/E (Sca-1) Recombinant Antibody	QA17A36	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 09/22/2021

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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