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Purified anti-mouse Lypd8 Antibody

Pricing & Availability
Clone
SA025A4 (See other available formats)
Regulatory Status
RUO
Other Names
Ly6/PLAUR domain containing 8
Isotype
Mouse IgG1, κ
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Product Citations
publications
SA025A4_PURE_Lypd8_Antibody_1_FC_110116
Mouse Lypd8-transfected cells where stained with purified Lypd8 (clone SA025A4, filled histogram) or purified mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG1 PE.
  • SA025A4_PURE_Lypd8_Antibody_1_FC_110116
    Mouse Lypd8-transfected cells where stained with purified Lypd8 (clone SA025A4, filled histogram) or purified mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG1 PE.
  • SA025A4_PURE_Lypd8_Antibody_2_IHCF_110116
    C57BL/6 mouse frozen colon section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 5 µg/ml of purified anti-mouse Lypd8 (clone SA025A4) overnight at 4°C, followed by 2.5µg of anti-mouse IgG Alexa Fluor® 488 (green) and Alexa Fluor® 594 anti-mouse CD326/Ep-CAM (clone G8.8) antibody (red) for two hours at room temperature. The image was captured with a 10X objective.
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152702 500 µg £262
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Description

Ly6/PLAUR domain containing 8 (Lypd8) is a 100-120 kD GPI-anchored glycoprotein and a member of the Ly6/PLAUR family. Lypd8 is highly expressed on epithelial cells from intestinal lumen, where it prevents bacterial invasion by inhibiting bacterial motility by binding to flagella.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Mouse Lypd8-transfected cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Verified
IHC-P - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 5.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone does not recognize Lypd8 from BALB/c mice.

Application References

(PubMed link indicates BioLegend citation)
  1. Okumura R, et al. 2016. Nature 532:117.
RRID
AB_2632691 (BioLegend Cat. No. 152702)

Antigen Details

Structure
GPI-anchored, glycoprotein, 100-120 kD.
Distribution

Epitelials cells in cecum and large intestine.

Function
Inhibits bacterial motility by binding to flagella.
Cell Type
Epithelial cells
Biology Area
Immunology, Innate Immunity
Antigen References

1. Okumura R, et al. 2016. Nature 532:117.
2. Loughner CL, et al. 2016. Hum. Genomics 10:10.

Gene ID
70163 View all products for this Gene ID
UniProt
View information about Lypd8 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 05/26/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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