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Purified anti-mouse MERTK (Mer) Antibody

Pricing & Availability
Clone
2B10C42 (See other available formats)
Regulatory Status
RUO
Other Names
Mer, Eyk, Nyk, nmf12
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
2B10C42_Pure_MERTK_Antibody_1_012816
Microglial cells from an adult mouse were enriched through a 37%/70% Percoll gradient and stained with purified mouse MERTK (clone 2B10C42)(top) or purified rat IgG2a, κ isotype control (bottom), followed by anti-rat IgG PE antibody, and subsequently stained with CX3CR1 Brilliant Violet 421™.
  • 2B10C42_Pure_MERTK_Antibody_1_012816
    Microglial cells from an adult mouse were enriched through a 37%/70% Percoll gradient and stained with purified mouse MERTK (clone 2B10C42)(top) or purified rat IgG2a, κ isotype control (bottom), followed by anti-rat IgG PE antibody, and subsequently stained with CX3CR1 Brilliant Violet 421™.
  • 2B10C42_Pure_MERTK_Antibody_2_012816
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151502 100 µg £182
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Description

MerTK (Mer) is a member of the TAM (TYRO3/AXL/MerTK) family. It is a transmembrane protein with two fibronectin type-III domains, two Ig-like C2-type domains, and one tyrosine kinase domain. MerTK is mainly expressed by macrophages, monocytes, and dendritic cells. Its ligands are LGALS3, TUB, TULP1, and GAS6. MerTK is involved in the regulation of TLR signaling, efferocytosis, phagocytosis, cell survival, macrophage migration, and the inhibition of inflammation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse MERTK extracellular domain
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. O'Dea KP, et al. 2020. J Extracell Vesicles. 9:1706708. PubMed
  2. Qiu Z, et al. 2022. Cancer Discov. 12:502. PubMed
RRID
AB_2566624 (BioLegend Cat. No. 151502)

Antigen Details

Structure
Member of the TAM (TYRO3/AXL/MerTK) family. Encodes a transmembrane protein, two fibronectin type-III domains, two Ig-like C2-type domains, and one tyrosine kinase domain.
Distribution

Macrophages, monocytes, and dendritic cells.

Function
Regulates TLR signaling, efferocytosis, phagocytosis, cell survival, and macrophage migration. Inhibits production of inflammatory cytokines.
Interaction
Grb2, LimD4, and Vav1.
Ligand/Receptor
LGALS3, TUB, TULP1, and GAS6.
Cell Type
Dendritic cells, Macrophages, Monocytes
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
Toll Like Receptors
Antigen References

1. Zagórska A, et al. 2014. Nat. Immunol. 15:920.
2. Toda S, et al. 2014. Blood 123:3963.
3. Chung WS, et al. 2013. Nature 504:394.
4. Carrera Silva EA, et al. 2013. Immunity 39:160.
5. Yi Z, et al. 2009. Blood 114:3191.

Gene ID
17289 View all products for this Gene ID
UniProt
View information about MERTK on UniProt.org
Go To Top Version: 1    Revision Date: 02/05/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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