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Purified anti-mouse MIF Antibody

Pricing & Availability
Clone
M3907B02 (See other available formats)
Regulatory Status
RUO
Other Names
Macrophage migration inhibitory factor (MMIF), Glycosylation inhibiting factor (GIF), GLIF, Phenylpyruvate Tautomerase, L-Dopachrome Tautomerase, L-Dopachrome Isomerase
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
M3907B02_PURE_MIF_Antibody_013117
  • M3907B02_PURE_MIF_Antibody_013117
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532502 500 µg £321
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Description

MIF (human macrophage migration inhibitory factor) is a non-glycosylated polypeptide expressed by multiple cell types, which include activated T cells, macrophages, eosinophils, epithelial cells, and endothelial cells. It is secreted by response to diverse inflammatory stimuli, and has been associated with a clear pro-inflammatory and proatherogenic role in multiple studies involving patients and animals. MIF also positively correlates with obesity as well as insulin resistance and contributes to adipose tissue inflammation by modulating ATM functions. Furthermore, MIF has been described as a protein that plays an essential role in both innate and acquired immunity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
In-house recombinant mouse MIF.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration of 3.0 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of MIF recombinant protein ranging from 10 to 0.16 ng/mL is recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance.

Application Notes

ELISA Capture: The purified M3907B02 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated Poly5240 antibody (Cat. No. 524004) as the detecting antibody.

Note: For testing mouse MIF in serum, plasma, cell culture supernatant, and cell lysate, BioLegend's LEGEND MAX™ Kits are specially developed and recommended.

RRID
AB_2686958 (BioLegend Cat. No. 532502)

Antigen Details

Structure
115 amino acids with a predicted molecular weight of approximately 12.5 kD.
Distribution

Activated T-cells, macrophages, eosinophils, epithelial cells, and endothelial cells.

Function
MIF has been associated with a clear pro-inflammatory and proatherogenic role in cardiovascular diseases. MIF also plays important roles in adipose tissue inflammation and innate and acquired immunity.
Interaction
Monocytes and macrophages, mesenchymal cells, and B-cells.
Ligand/Receptor
CD44/CD74, CXCR4, CXCR2, and CXCR7.
Cell Type
T cells, Macrophages, Eosinophils, Epithelial cells, Endothelial cells
Biology Area
Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Immunology, Innate Immunity, Signal Transduction
Molecular Family
Cytokines/Chemokines
Antigen References

1. Zhou H, et al. 2009. PLoS One. 4:e6087.
2. Potolicchio I, et al. 2003. J. Biol. Chem. 278:30889.
3. Meyer-Siegler K and Iczkowski K. 2005. BMC Cancer 5:73.
4. Babu SN, et al. 2012. Asian Pac. J. Cancer Prev. 13:1737.
5. van der Vorst EP, et al. 2015. Front Immunol. 6:373.
6. Kim BS, et al. 2015. Exp. Mol. Med. 47:e161.
7. Sanchez-Zamora YI, et al. 2014. J. Diabetes Res. 2014:804519.
8. Gilliver SC, et al. 2011. Exp. Dermatol. 20:1.
9. Bach JP, et al. 2008. Oncology 75:127.

Gene ID
17319 View all products for this Gene ID
UniProt
View information about MIF on UniProt.org
Go To Top Version: 0    Revision Date: 04/10/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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  • Purified anti-mouse MIF

    M3907B02_PURE_MIF_Antibody_013117
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