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Purified anti-mouse NGAL (Lipocalin-2) Antibody

Pricing & Availability
Clone
M047A10 (See other available formats)
Regulatory Status
RUO
Other Names
Lipocalin 2, Oncogene 24p3, Neutrophil Gelatinase-Associated Lipocalin, Siderocalin LCN2, 25 kD Alpha-2-Microglobulin-Related Subunit Of MMP-9, p25, MSFI, Migration-Stimulating Factor Inhibitor, HNL
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
M047A10_PURE_NGAL_Antibody_021417
  • M047A10_PURE_NGAL_Antibody_021417
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532202 500 µg £398
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Description

NGAL (lipocalin-2) is a lipocalin family protein that plays important roles in innate immunity, inflammation, and atherosclerosis. Mouse NGAL can be detected in the lung, spleen, uterus, vagina, and epididymis. It is a critical component of innate immunity due to its ability to bind iron-siderophore complexes and limit bacterial growth. It is also produced by injured epithelia and is one of the most promising new markers for renal epithelial injury and cardiovascular disease (CVD).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
In-house recombinant mouse NGAL
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA - Quality tested
IP - Verified
Recommended Usage

Each lot of this antibody is quality control tested by ELISA. For ELISA capture applications, a concentration of 1.5 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of NGAL recombinant protein ranging from 1000 to 15.6 pg/mL is recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance.

Application Notes

The purified M047A10 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated M0417A8 antibody as the detecting antibody.

For testing mouse NGAL in cell culture supernatant, cell lysate, serum, plasma, urine, and other biological fluids, BioLegend's specially developed LEGEND MAX™ Kits are recommended.

RRID
AB_2650711 (BioLegend Cat. No. 532202)

Antigen Details

Structure
195 amino acid monomer with a predicted molecular weight of approximately 23 kD.
Distribution

Secreted by epithelial cells, macrophages, neutrophils; increased levels have been observed in plasma, serum, and urine in various conditions, such as metastatic breast and colorectal cancer, acute kidney injury, pancreatitis and preeclampsia.

Function
Apoptosis, innate immunity, renal development.
Interaction
Disulfide-linked with MMP9.
Ligand/Receptor
Apoptosis, innate immunity, renal development.
Cell Type
Epithelial cells, Macrophages, Neutrophils
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Innate Immunity, Signal Transduction
Antigen References

1. Iqbal N, et al. 2013. Expert Opin.Med. Diagn. 7:209.
2. Singer E, et al. 2013. Acta. Physiol. (Oxf) 207:663.
3. Palazzuoli A, et al. 2012. Clin. Chim. Acta. 413:1832.
4. Flo TH, et al. 2004. Nature 432:917.
5. Goetz DH, et al. 2002. Mol. Cell. 10:1033.
6. Leung L, et al. 2012. PLoS One 7:e46677.
7. Kjeldsen L, et al. 1993. J. Biol. Chem. 268:10425.

Gene ID
16819 View all products for this Gene ID
UniProt
View information about NGAL on UniProt.org
Go To Top Version: 0    Revision Date: 03/27/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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  • Purified anti-mouse NGAL (Lipocalin-2)

    M047A10_PURE_NGAL_Antibody_021417
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