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Purified anti-mouse PD-1H (VISTA) Antibody

Pricing & Availability
Clone
MH5A (See other available formats)
Regulatory Status
RUO
Other Names
PD-1 homolog, Dies1, B7-H5
Isotype
Armenian Hamster IgG
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Product Citations
publications
1-MH5A_Pure_1_040412
C57BL/6 mouse splenocytes were stained with CD8 APC and purified PD-1H (clone MH5A) (top) or Armenian Hamster IgG isotype control (bottom), followed by anti-Armenian Hamster IgG-PE.
  • 1-MH5A_Pure_1_040412
    C57BL/6 mouse splenocytes were stained with CD8 APC and purified PD-1H (clone MH5A) (top) or Armenian Hamster IgG isotype control (bottom), followed by anti-Armenian Hamster IgG-PE.
  • 2-MH5A_Pure_2_040412
  • 3-MH5A_Pure_PD-1H_Antibody_3_092018
    Balb/C mouse frozen spleen tissue slices were fixed with 4% PFA for ten minutes and blocked with 5% FBS for 30 minutes. Then, the tissue was stained with 10 µg/mL of Purified anti-mouse PD-1H (VISTA) antibody (clone MH5A) overnight at 4°C. On the next day, tissue was incubated with Alexa Fluor® 647 Goat anti-hamster (Armenian) IgG Antibody (clone Poly4055, red) and Alexa Fluor® 594 anti-mouse CD3ε Antibody (Clone 17A2, green) for an hour at room temperature. Nuclei were counter-stained with DAPI (blue). The image was scanned with a 10X objective and stitched with MetaMorph® software.
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143702 100 µg £101
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Description

PD-1H, also known as VISTA, is a 309 aa type I transmembrane protein, composed of seven exons. PD-1H has one Ig-V like domain, and its sequence is similar to the Ig-V domains of the members of CD28 and B7 families. PD-1H is expressed by a subset of T cells, macrophages, dendritic cells, neutrophils, and NK cells. It has been proposed that PD-1H can be useful to modulate the host immune response to allogeneic transplants. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
PD-1H- IgG Fc fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Verified
WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. For immunohistochemistry on frozen tissue sections, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: inhibition of graft vs host disease (GVHD), Western blotting, and immunohistochemical staining of paraffin embedded tissue sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Flies DB, et al. 2011. J. Immunol. 187:1537.
Product Citations
  1. Wei SC et al. 2017. Cell. 170(6):1120-1133 . PubMed
  2. Wei W, et al. 2022. mSystems. 7:e0046922. PubMed
RRID
AB_11150598 (BioLegend Cat. No. 143702)

Antigen Details

Structure
Type I transmembrane protein, member of the CD28 family
Distribution

Macrophages, dendritic cells, neutrophils, NK cells, naïve and activated T cells

Function
Negative regulator of the immune response
Cell Type
Dendritic cells, Macrophages, Neutrophils, NK cells, T cells
Biology Area
Immunology, Inhibitory Molecules
Antigen References

1. Flies DB, et al. 2011. J. Immunol. 187:1537.
2. Wang Li, et al. 2011. J. Exp Med. 208:577.

Gene ID
74048 View all products for this Gene ID
UniProt
View information about PD-1H on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 09/20/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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