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Purified anti-mouse PIR-A/B Antibody

Pricing & Availability
Clone
6C1 (See other available formats)
Regulatory Status
RUO
Other Names
PIRA1, PIR-B Leukocyte immunoglobulin-like receptor 3, LIR-3
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
6C1_Pure_051812
C57BL/6 mouse splenocytes were stained with purified PIR-A/B (clone 6C1) (filled histogram) or rat IgG1, κ isotype control (open histogram), followed by anti-rat IgG PE.
  • 6C1_Pure_051812
    C57BL/6 mouse splenocytes were stained with purified PIR-A/B (clone 6C1) (filled histogram) or rat IgG1, κ isotype control (open histogram), followed by anti-rat IgG PE.
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144101 50 µg £77
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Description

Paired-immunoglobulin-like receptor (PIR) is a type I transmembrane protein, containing six Ig-like domains. Two isoforms (PIR-A and PIR-B) have been reported for this molecule. PIR-A/B is expressed on B cells, mast cells, macrophages, granulocytes, eosinophils, and dendritic cells. PIR-A requires association with FcRγc for its cell surface expression and to act as an activating receptor. The ligand for PIR-A is MHC class I. PIR-B is an inhibitory receptor with 3 ITIM motifs on its cytoplasmic tail; when phosphorylated, they recruit the tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2. MHC class I, Nogo, MAG, and OMgp are the ligands for PIR-B.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant extracellular domains 1 and 2 of PIR-A
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F, WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications for the relevant formats include: Western Blotting1 and immunohistochemical staining of frozen tissue sections3.

Application References

(PubMed link indicates BioLegend citation)
  1. Kubagawa H, et al. 1999. J. Exp. Med. 189:309. (WB)
  2. Nakamura A, et al. 2004. Nat. Immunol. 5:623. (FC)
  3. Masuda K, et al. 2005. EMBO J. 24:4052. (IHC)
Product Citations
  1. Shang P, et al. 2023. Nat Commun. 14:2615. PubMed
RRID
AB_11203714 (BioLegend Cat. No. 144101)

Antigen Details

Structure
Transmembrane proteins, six Ig-like domains. PIR-A associates with FcRγc, doesn't have functional motifs on its cytoplasmic domain. PIR-B contains 3 ITIM motifs on its cytoplasmic domain.
Distribution

B cells, macrophages, dendritic cells, mast cells, eosinophils.

Interaction
PIR-A: FcRγc
PIR-B: Lyn, PTPN6/SHP-1, PTPN11/SHP-2
Ligand/Receptor
MHC class I, Nogo, MAG, OMgp.
Cell Type
B cells, Dendritic cells, Eosinophils, Macrophages, Mast cells, Neutrophils
Biology Area
Cell Biology, Immunology, Signal Transduction
Molecular Family
MHC Antigens, Protein Kinases/Phosphatase
Antigen References

1. Matsushita H, et al. 2011. J. Biol. Chem. 286:25739.
2. Arita K, et al. 2011. J. Immunol. 186:7050.
3. Takai T, et al. 2011. J. Biomed. Biotechnol. 2011:275302.
4. Nakamura A, et al. 2004. Nat. Immunol. 5:623.

Gene ID
18722 View all products for this Gene ID 18733 View all products for this Gene ID
UniProt
View information about PIR-A/B on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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